在吸毒人群中应用ELISA检测尿液及血液标本中HIV-1抗体结果分析

Analysis on Detecting HIV - 1 Antibody in Paired Urine and Serum Specimens from Drug Users by ELISA

目的 比较利用吸毒人群的尿液和血液标本检测HIV-1抗体结果的一致性。方法 对某市强制戒毒所273例吸毒者分别采集其尿液和血液标本,应用ELISA方法分别采用尿液和血液初筛试剂检测尿液和血液标本中的HIV-1抗体。结果273例中有94例血液标本的HIV-1抗体为阳性,其平行尿液标本中有93例HIV-1抗体为阳性。分析结果表明应用尿液、血液标本检测HIV-1抗体方法的一致性为99.6％。结论 采用尿液试剂进行HIV-1抗体检测结果是可靠的,而且是值得大范围推广的。

Objective To compare the consistency of the results from detecting HIV - 1 antibody in the Paired urine and serum specimens from drug users by ELISA. Methods The paired urine and serum samples from 273 drug users detained at a detoxification unit were collected and, the HIV - 1 antibodies in the samples of them were screened by urine and serum ELISA kits, respectively. Results Of 273 serum samples, 94 ones showed positive reaction and, among 94 counterpart urine samples, 93 ones also appeared positive reaction. Taking the results together, the consistent rate of HIV- 1 antibody screened by urine and serum ELISA kits was 99.6% . Conclusion The urine ELISA kit, which screened HIV - 1 antibody of urine showing almost the same results tested by serum ELISA kit, is reliable. It is proposed that urine ELISA be introduced in many fields.