假型逆转录病毒高效介导人乳腺癌细胞基因转移

High efficiency gene transfer into breast cancer cells by pseudotyped retroviral vector

目的 :探索假型逆转录病毒MuLV/VSV G在乳腺癌细胞基因转导的效率 ,为乳腺癌细胞基因转导找寻一种高效的载体。方法 :将含报道基因的假型逆转录病毒MuLV/VSV G转导入人乳腺癌MDA MB 435细胞 ,观察其转导效率 ,并与MuLV的转导效率进行比较。结果 :MuLV/VSV G的转导效率达 (92± 12 ) % ,MuLV的转导效率为 (2 4±5 ) % ,前者较MuLV转导效率提高 3.8倍。结论 :假型逆转录病毒MuLV/VSV G可作为一种高效的载体广泛应用于乳腺癌的基因治疗研究。

Objective: To investigate the efficiency of gene transfer mediated by pseudotyped retroviral vector in breast cancer,and to provide an useful vector for breast cancer gene therapy. Methods:We used a MuLV vector pseudotyped with the vesicular stomatitis virus glycoprotein(VSV G)envelope to transduce breast cancer cells MDA MB 435, To compare the transfer efficiency with MuLV.Results:The transfer efficiency is (92±12)% by using MuLV/VSV G vector, (24±5)% by MuLV vector. The transfer efficiency of MuLV/VSV G is 3.8 times that of MuLV vector. Conclusion: Our results suggest that pseudotyped retroviral vector MuLV/VSV G is a kind of high efficiency gene transfer vector in breast cancer cells. [