肝癌患者血清IL-10,IL-12和TNFα水平的变化

Level change of IL-10, IL-12 and TNFα in the serun of patients with hepatocellular carcinoma

目的 :探讨细胞因子IL 10 ,IL 12和TNFα与原发性肝癌之间的关系及临床意义。方法 :采用ELISA法检测了 40例原发性肝癌患者 ,36例肝炎后肝硬化患者和 33名健康人的血清中IL 10 ,IL 12和TNFα的浓度。结果 :在肝硬化患者三项指标较健康人显著升高 (P <0 .0 1) ,而肝癌患者与健康人比较除IL 10升高外 ,IL 12和TNFα差异无显著性 (P >0 .0 5 ) ;IL 10在肝癌肿块直径 >5 .0cm的患者中比直径 <5 .0cm的患者显著升高 (P <0 .0 5 ) ,而IL 12和TNFα则显著降低 (P <0 .0 5 )。结论 :原发性肝癌患者免疫功能异常可能与IL 10的增高有关 ;IL 12 ,TNFα水平降低和IL 10水平升高有助于鉴别肝硬化结节和肝癌的临床诊断及提示癌变倾向。

Objective: To study the relationship and clinical significance between level change of serum Interleukin 10 (IL 10), interleukin 12 (IL 12), tumor necrosis factor α (TNFα) and primary hepatic carcinoma (PHC). Methods: serum IL 10, IL 12, TNFα in 40 patients with PHC, 36 cases with hepatocirrhosis and 33 normal controls were measured with ELISA. Results: The higher serum levels of IL 10, IL 12, TNFα were observed in patients with hepatocirrhosis than in controls (P<0.01), although IL 12, TNFα in the serum of patients with PHC were in the proximity of that of controls (P>0.05), the IL 10 level was significantly higher than that of control (P<0.01), further more, IL 10 being opposed to IL 12, TNFα is higher in patients with tumor above 5.0?cm in diameter than below 5.0?cm, IL 12 and TNFα were declined and IL 10 increased with tumor enlargement. Conclusion: The immune dysfuncton in patients with PHC may be related to level increase of IL 10. The level decrease of IL 12, TNFα and increase of IL 10 is helpful to clinical differential diagnoses between cirrhotic nodule and PHC, and this suggests cancerous tendency. [