载脂蛋白E基因多态性与冠心病的关系

Relationship between apolipoprotein E polymorphism and coronary heart disease

目的 研究载脂蛋白E(ApoE)基因多态性与冠心病发病及对血脂代谢影响的关系。 方法 对 71例冠心病患者 (CHD组 )和 6 9例正常对照者 (对照组 )的静脉全血白细胞提取DNA ,应用PCR ,HhaI酶切 ,用 12 %聚丙烯酰胺凝胶电泳 ,检测出其ApoE基因型及等位基因分布频率。 结果 CHD组及对照组均检出 4种基因型 ,分别是 :E2 / 3 ,E3/ 3 ,E2 / 4 ,E3/ 4 型。CHD组与对照组比较 :E3/ 4 基因型频率有显著性差异 (P <0 0 5 ) ;E4 等位基因频率有显著性差异 (P <0 0 1)。结论 ApoE基因多态性同冠心病发病有关 ,E4 等位基因是影响冠心病发病的重要遗传因素之一。

Objective To study the effect of apolipoprotein E(ApoE) polymorphism on serum lipids and the relationship between apolipoprotein E polymorphism and coronary heart disease(CHD).Methods Polymerize chain reaction(PCR) and HhaI digestion were used for the detection of apolipoprotein E genotype on 71 patients with CHD and 69 control subjects.Results The frequencies of apolipoprotein E 4 allele and E 3/4 genotype were obviously increased in CHD group than in control group(P<0 01,P<0 05).Conclusion The apolipoprotein E polymorphism is related to the occurrence of coronary heart disease;apolipoprotein E 4 allele is one of the most important hereditary factor for the occurrence of CHD.