清脉颗粒醇沉工艺的正交优选

Optimization of Alcohol Precipitation Technology for Qingmai Granules by Orthogonal Test

目的：优选清脉颗粒的醇沉工艺。方法：选取药液相对密度,醇沉浓度及醇沉时间为考察因素,以干浸膏得率,薯蓣皂苷元苷含量为考察指标,采用正交试验优选醇沉工艺。结果：优选的醇沉工艺为药液浓缩至相对密度1.13 - 1.18 g·;ml-1,加乙醇至乙醇体积分数为60%,醇沉24 h。结论：优选的工艺稳定可行,为清脉颗粒的临床应用提供试验依据。

Objective：To optimize the alcohol precipitation technology of Qingmai granules. Methods：The relative density of liq-uor,the concentration and time of alcohol precipitation were chosen as the factors,the yield of dry extract and content of diosgenin as the indices,the alcohol precipitation technology was optimized by orthogonal test. Results：The optimal alcohol precipitation technology was as follows：the extraction liquid was concentrated till the relative density was 1. 13-1. 18 g·;ml-1 ,ethanol was added until the concen-tration was up to 60% with the alcohol precipitation time of 24h. Conclusion：The optimized technology is stable,reasonable and feasi-ble,which can provide experimental basis for the clinical application of Qingmai granules.