HPLC法测定灵芝二维甲硫氨酸胶囊中甲硫氨酸、维生素B_1及维生素B_2含量

Determination of Methionine,Vitamin B_1 and Vitamin B_2 in Lingzhi Erwei Methionine Capsules by HPLC

目的：建立用HPLC法同时测定灵芝二维甲硫氨酸胶囊中甲硫氨酸、维生素B1及维生素B2含量的方法。方法：采用Ultimate？ AQ-C18色谱柱（250 mm ×4.6 mm,5μm,Welch公司）,流动相：甲醇-0.07 mol·L-1庚烷磺酸钠溶液（加14 ml三乙胺并加水至1000 ml,用磷酸调节pH至3.5）,线性梯度洗脱,检测波长为220 nm,流速：1.0 ml·min-1。结果：甲硫氨酸、维生素B1、维生素B2分别在0.5270~1.5100 mg·ml-1（r=0.9999）、0.04552~0.13656 mg·ml-1（r=0.9999）、0.01010~0.06058 mg·ml-1（r =0.9999）范围内线性关系良好,平均回收率分别为100.5%、97.7%、101.5%,RSD 分别为0.5%、1.0%、1.4%（n=9）。结论：本法操作简便、结果准确、可靠,可用于灵芝二维甲硫氨酸胶囊中甲硫氨酸、维生素B1、维生素B2的含量测定。

Objective：To establish an HPLC method for the simultaneous determination of methionine, vitamin B1 and vitamin B2 in Lingzhi Erwei methionine capsules. Methods：The determination was performed on a Ultimate？ AQ-C18 column（250 mm × 4. 6 mm, 5 μm,Welch Inc. ） with the detection wavelength at 220 nm. The mobile phase was composed of methanol and 0. 07 mol·L-1 sodium heptanesulfonate solution （14 ml triethylamine diluted with water to1 000ml, adjusting pH to 3. 5 with diluted orthophosphoric acid） with gradient elution, and the flow rate was 1. 0 ml·min-1. Results： Methionine, vitamin B1 and vitamin B2 was in good linearity within the range of 0.527 0-1.510 0 mg·ml-1 - （r =0.999 9）, 0.045 52-0.136 56 mg·ml-1 （r =0.999 9） and 0.010 10-0. 060 58 mg·ml-1（r=0. 999 9）, respectively. The mean recovery was 100. 5%, 97. 7% and 101. 5% with RSD of 0. 5%, 1. 0%and 1. 4%（n=9） accordingly. Conclusion：The method is simple, accurate, reliable and appropriate in the simultaneous determina-tion of methionine,vitamin B1 and vitamin B2 in Lingzhi Erwei methionine capsules.