白及幼根组织培养技术研究

Study on Tissue Culture Techniques for Radicle of Bletilla Striata

目的:为白及组培快繁寻求最佳部位及最佳培养基配比。方法:研究不同激素浓度和配比对白及不同外植体诱导分化、增殖及生根的影响。结果:幼根最容易诱导出丛生芽,其最佳培养基为1/2MS+1.0 mg·L-16-BA+2.0 mg·L-1NAA;丛生芽增殖最佳培养基为MS+1.0 mg·L-16-BA+0.05 mg·L-1NAA;生根培养基以1/2MS+0.5 mg·L-1NAA为最佳。结论:建立了白及组培快繁体系。

Objective：To seek the best part and optimal culture medium for the tissue culture of Bletilla striata. Methods： The effects of hormone concentration and proportion on the induced differentiation, propagation and rootage of Bletilla striata were investiga-ted. Results：The radicle of Bletilla striata was the best part to induce the clustered shoots with the optimal culture medium of 1/2MS＋1. 0 mg·L-1 6-BA＋2. 0 mg·L-1 NAA. The best hormone concentration for inducing the clustered shoots was MS＋1. 0 mg·-1 L 6-BA＋0. 05 mg·L-1 NAA, and the optimal rooting medium was 1/2MS＋0. 5 mg·L-1 NAA. Conclusion： The tissue culture system for Bletilla striata is established.