高效液相色谱双波长检测法测定苏合香丸中苯甲酸与肉桂酸的含量

Determination of Benzoic Acid and Cinnamic Acid in Suhexiang Pills by HPLC with Dual Wavelength Detection

目的：建立高效液相色谱双波长检测法同时测定苏合香丸中苯甲酸和肉桂酸含量。方法：采用HPLC Waters Sun Fire TM C18柱（150 mm ×4.6 mm,5μm）；以0.1%醋酸水溶液-甲醇（60∶40）为流动相,流速：1.0 mL·min-1；检测波长：0~18 min时228 nm（苯甲酸）,18.1~35 min时285 nm（肉桂酸）；进样量10μL；柱温：室温。结果：苯甲酸和肉桂酸的线性范围分别为1.0~50.0μg·ml-1（r=0.9997）,0.2~10.0μg·ml-1（r=0.9999）；平均回收率分别为96.88%（RSD=1.6%,n=9）和99.35%（RSD=1.7%,n=9）。结论：苯甲酸和肉桂酸的线性范围分别为1.0~50.0μg·ml-1（r=0.9997）,0.2~10.0μg·ml-1（r=0.9999）；平均回收率分别为96.88%（RSD=1.6%,n=9）和99.35%（RSD=1.7%,n=9）。

Objective：To establish an HPLC method with dual wavelength detection for the determination of benzoic acid and cin-namic acid in Suhexiang pills. Methods：A Waters Sun Fire TM C18 column（150 mm ×4. 6 mm,5 μm） was adopted with 0. 1% ace-tic acid-methanol（60∶40） as the mobile phase at a flow rate of 1. 0 ml·min-1 . The detection wavelength was set at 228nm for benzoic acid（0-18min） and 285nm for cinnamic acid （18. 1-35min）. The injection volume was 10μl, and the column temperature was room temperature. Results：The linear range of benzoic acid and cinnamic acid was 1. 0-50. 0 μg·ml-1（r=0. 999 7） and 0. 2-10. 0 μg· ml-1, respectively（r=0. 999 9）. The average recovery was 96. 88%（RSD=1. 6%, n=9） and 99. 35%（RSD=1. 7%, n=9）, re-spectively. Conclusion：The method is accurate, fast and simple in the determination of benzoic acid and cinnamic acid in Suhexiang pills.