D7S817,D18S865位点的群体遗传学研究

Genetic analysis of the STR system D7S817 and D18S865 loci in Chinese HAN and DONGXIANG populations

目的 调查D7S817、D18S86 5两个STR位点的遗传多态性 ,获得群体遗传学基本数据。 方法 采用PCR和PAG垂直电泳技术、银染显色方法。结果 D7S817位点在成都汉族群体中发现 9个等位基因 ,2 3种基因型 ,杂合度为 0 .738,个人识别机率为 0 .931。在甘肃东乡族群体中发现 8个等位基因 ,2 0种基因型 ,杂合度为 0 .75 2 ,个人识别机率为 0 .917。D18S86 5位点在成都汉族群体中发现 7个等位基因 ,17种基因型 ,杂合度为 0 .72 ,个人识别机率为 0 .90 6 ;在甘肃东乡族群体中发现 6个等位基因 ,15种基因型 ,杂合度为 0 .814,个人识别机率为 0 .898。基因型频率分布符合Hardy -Weinberg平衡定律。等位基因频率的分布在 2个群体之间无显著性差异。 结论 D7S817、D18S86 5位点的扩增效率高 ,重复性好 ,个人识别能力强 ,在法医学个人识别和亲子鉴定应用中有较高的价值。

Objective To study the genetic polymorphisms of two new STR loci D7S817, D18S865 in Chinese Han and Dongxiang populations. Methods EDTA-blood samples were collected from unrelated individuals and DNA samples were extracted by “CHELEX-100” method. The products of the polymerase chain reaction (PCR) were analyzed by nondenaturing polyacrylamide gels electrophoresis and silverstaining. Results On D7S817 locus, 9 alleles and 23 genotypes were found in Wuhan area Han population, 8 alleles and 20 genotypes were found in Ganshu province Dongxiang population. Therewith, at D18S865 locus, 7 alleles and 17 genotypes were identified in Wuhan area Han population, 6 alleles and 15 genotypes were identified in Ganshu province Dongxiang population. The heterozygosity and discrimination powers (DP) of D7S817 were 0.738 and 0.931respectively, in Wuhan Han population; 0.752 and 0.917 respectively, in Ganshu province Dongxiang population. The heterozygosity and DP of D18S865 were 0.72 and 0.906 respectively, in Wuhan Han population; 0.814 and 0.898 respectively in Ganshu Dongxiang population. The distribution of genotypes frequency in both loci were accorded with Hardy-Weinberg equilibrium. There were no significantly differences on distribution of allele frequency between the two populations. Conclusion These results suggest that STR system D7S817 and D18S865 loci are useful markers in forensic haemogenetics.