雾化吸入γ-干扰素对免疫低下大鼠某些细胞因子的影响

Effect of IFN-γ inhalation on some cytokines of immunocompromised rats

目的 研究雾化吸入γ 干扰素 (IFN γ)对免疫低下大鼠细胞因子的影响。方法 醋酸考的松皮下注射并气管内注射白色念珠菌复制大鼠免疫低下肺感染模型 ,检测雾化吸入IFN γ 1、3、7d的免疫低下大鼠和对照组大鼠肺泡巨噬细胞 (AM)培养上清中TNF α活性和IL 1β、IL 6水平 ,支气管肺泡灌洗液 (BALF)IFN γ、TNF α活性 ,肺组织IFN γ、TNF α、IL 1β、IL 6表达 ,以及血清IFN γ活性和IL 1β、IL 6水平。 结果 吸入IFN γ组大鼠AM培养上清中TNF α的活性和IL 1β、IL 6水平显著高于对照组大鼠。吸入IFN γ组大鼠BALF中IFN γ、TNF α活性高于对照组大鼠 (第 7天TNF α除外 )。吸入IFN γ组灌菌后第 7天肺组织IFN γ和IL 1β的表达高于对照组 ,TNF α表达低于对照组 ,IL 6表达二组无显著差异。吸入IFN γ组大鼠血清IFN γ活性和IL 1β、IL 6水平 ,除第 3天IL 1β外 ,与免疫低下大鼠组相比差异不显著。 结论 雾化吸入IFN γ可明显提高肺内IFN γ、TNF α、IL 1β、IL 6水平 ,但对相应血清细胞因子无明显影响。

Objective To study the effect of IFN γ inhalation via aerosol on cytokines of the immunocompromised rats. Methods Immunocomprised rat model was established with cortisol acetate injection for 14 d and then Candida albicans fluid was injected by tracheal for establishing am immuno comprised with pulmonary infection model. IFN γ was inhaled with aerosol 1 d before the bacterium injection and then for 1, 3 and 7 d respectively. The activity of TNF α, and the levels of IL 1β and IL 6 in the supernatant of the cultured alveolar macrophage(AM), the activity of IFN γ and TNF-α in bronchial alveolar lavage fluid (BALF), the expressions of IFN γ,TNF-α, IL 1β, and IL 6 of the lung tissues, the level of IFN γ,IL 1β, and IL 6 in the serum were investigated. Results The activity of TNF-α, and the levels of IL 1β and IL 6 in the culture supernatant of the AM of the rats treated with IFN γ were significantly higher than those of the control. The activity of IFN γ and TNF α in BALF was higher in the IFN γ inhaled rats than in the control (except the activity of TNF α on the 7th day). The expressions of IFN γ and IL 1β in lung tissues was higher in the rats treated with IFN γ than in the control. The expression of TNF α in the rats treated with IFN γ was less than that in the control rats. The expression of IL 6 had no difference between 2 groups. And no difference was found in the activity of IFN γ, and the levels of IL 1β and IL 6 in the serum between 2 groups(except IL 1β on the 3rd day). Conclusion Administration of IFN γ via aerosol can obviously increase the activity or levels of some cytokines in the lung of the immunocompromised rats, but has no effect on them in serum of the immunocompromised rats.