急性肺损伤早期肺表面活性蛋白A、B含量的变化

Alterations of surfactant protein A and B in the early stage of acute lung injury in rats

目的 探讨油酸性急性肺损伤早期表面活性蛋白A、B(SP A、SP B)含量的变化。方法 复制油酸性大鼠急性肺损伤模型 ,采用WesternBlot和ELISA术检测支气管肺泡灌洗液 (BALF)中SP A、SP B的含量。结果 BALF中SP A、SP B的含量在急性肺损伤后 1h就出现明显降低 (P <0 .0 1) ,并在 6h内维持较低水平。结论 急性肺损伤早期即出现SP A、SP B含量下降 ,同时 ,本研究所建立的表面活性蛋白含量监测方法灵敏度高 ,特异性好 ,可供实验研究采用。

Objective To study the alterations of surfactant protein A (SP A) and B (SP B) in rats in the early stage of acute lung injury induced by oleic acid. Methods An acute lung injury model was established by injecting oleic acid into the superior vena cava of rats and the levels of SP A and SP B in branchoalveolar lavage fluid (BALF) were determined with Western Blot and ELISA. Results The levels of SP A and SP B in BALF were significantly decreased in the injured group 1 h after acute lung injury ( P <0.01) and remained low in the next 6 h. Conclusion The levels of SP A and SP B were decreased in the early stage of acute lung injury. The methods used for detecting SP A and SP B we adopted are specific and sensitive, and might be helpful for other experimental study.