胰腺癌细胞TM4SF1的表达及其对细胞增殖、迁移及侵袭能力的影响

Expression of TM4SF1 and its effect on proliferation, migration and invasion in pancreatic cancer cells

目的 检测5株人胰腺癌细胞株中TM4SF1 mRNA的表达,探讨TM4SF1基因对癌细胞增殖、迁移及侵袭能力的影响.方法 采用实时PCR方法检测人胰腺癌细胞株MPanc96、MiaPaCa-2、HPAC、PANC1、AsPC-1的TM4SF1 mRNA表达,并与人正常胰腺导管上皮细胞（HPDE）的TM4SF1mRNA表达进行比较.应用RNA干扰方法将靶向TM4SF1的siRNA及阴性对照siRNA瞬时转染MPanc96、MiaPaCa-2细胞.采用MTS法检测转染细胞的增殖能力,Transwell小室法检测细胞的迁移及侵袭能力.结果 胰腺癌细胞株MPanc96、MiaPaCa-2、PANC1、AsPC-1、HPAC的TM4SF1 mRNA相对表达量分别为1.205±0.073、1.096±0.260、1.382±0.075、1.374±0.363、0.744±0.096,均显著高于HPDE的0.020±0.003（F =22.26,P＜0.01）.与转染阴性对照siRNA的细胞比较,TM4SF1基因表达沉默的MPanc96、MiaPaCa-2细胞的增殖无显著变化,但细胞的迁移力分别降低（62.5±7.6）％、（72.8±4.0）％,侵袭能力分别下降（69.5±5.7）％、（78.6±6.3）％.结论 TM4SF1在人胰腺癌细胞中高表达,并增强胰腺癌细胞的迁移和侵袭能力.

Objective To determine the expression of TM4SF1 mRNA in 5 human pancreatic cancer cell lines, and investigate its effect on the proliferation, migration and invasion of pancreatic cancer cells. Methods The expression of TM4SF1 mRNA in MPanc96, MiaPaCa-2, PANC1, AsPC-1, HPAC cells was determined by qRT-PCR, and the results were compared with that of human pancreatic ductal epithelial （HPDE） cells. RNA interference method was used to transiently transfect siRNA targeting at TM4SF1 and negative control siRNA into MPane96, MiaPaCa-2 cells. The proliferation of ceils were measured by MTS method, and migration and invasion of cells were determined by Transwell. Results The expression levels of TM4SF1 mRNA in pancreatic cancer cell lines MPanc96, MiaPaCa-2, PANC1, AsPC-1 and HPAC were 1. 205 ：i： 0.073, 1. 096 ± 0.260, 1. 382 ± 0. 075, 1. 374 ± 0.363 and 0.744 ± 0.096, which were significantly highly than that in HPDE （0.020 ± 0.003, P 〈 0.01 ）. Compared with cells transfected with negative control siRNA, the proliferation of MPanc96 and MiaPaCa-2 cells transfected with siRNA targeting at TM4SF1 was not significantly changed, but the migration abilitiy was decreased by （62.5 ± 7.6） % and （ 72.8 ± 4.0 ） %, and invasion abilitiy was decreased by （69.5 ± 5.7 ） % and （78.6 ± 6.3 ） %. Conclusions TM4SF1 is highlyexpressed in pancreatic cancer cells and appears to promote the migration and invasion abilities of the cancer cells.