扁平苔藓患者血清及唾液中IL-10的表达及相关性研究

Expression and correlation of IL-10 in serum and saliva of patients with oral lichen planus

目的:探讨口腔扁平苔藓(OLP)患者唾液及血清中白细胞介素-10(IL-10)的表达情况及其相关性,以进一步了解OLP患者唾液代替血液作为生物学样本来研究OLP中IL-10的可行性。方法:实验分3组:OLP糜烂组15例,OLP非糜烂组15例,正常对照组15例。采用ELISA法分别检测3组对象血清和唾液中IL-10的含量,对结果进行统计分析。结果:OLP糜烂组血清中IL-10水平为(30.11±3.02)pg/mL,明显高于非糜烂组(19.03±3.33)pg/mL和正常对照组(16.43±2.19)pg/mL,差异有统计学意义(P<0.05);OLP糜烂组唾液中IL-10平均水平为(10.05±3.12)pg/mL明显高于非糜烂组(5.50±1.1)pg/mL和正常对照组(4.47±1.7)pg/mL,差异有统计学意义(P<0.05);两实验组血清和唾液中IL-10水平成正相关,(r=0.731,P<0.01)。结论:实验组血清和唾液中IL-10水平高度相关,可以通过检查唾液替代检查血液来研究OLP中IL-10的水平。

Objective：Study on oral lichen planus（OLP） in patients with salivary and serum interleukin -10（IL-10） ex- pression and their correlation,In order to further understand the feasibility of IL-10 in saliva of patients with OLP instead of blood as a biological sample to study of oral lichen planus Method：The experiments were divided into three groupsOLP ero- sion Group 15,OLP non erosive Group 15,control group 15.The content of IL-10 of serum and saliva of three groups of ob- jects were measured by ELISA,and analyze the results. Result：The level of IL-10 in serum of OLP erosion group for （30.11＋ 3.02）pg/mL, significantly higher than that in non erosive group （19.03±3.33） pg/ml and normal control group （16.43±2.19） pg/mL, the difference was statistically significant （P 〈0.05）;IL-10 OLP erosion group saliva average （10.05±3.12）pg/mL was significantly higher than non erosion group （5.50±1.1）pg/mL and the normal control group （4.47±1.7）pg/mL,the difference was statistically significant （P 〈0.05）;the level of IL-10 into two experimental groups in serum and saliva of positive correla- tion,（r=0.731,P 〈0.01）. Conclusion：The experimental group in serum and saliva were highly correlated with IL-10 level,can be studied in OLP IL-10 by examining the saliva instead of blood level.