摘要
为调查武汉市美洲大蠊感染蠊缨滴虫(Lophomomas blattarum)的情况,解剖镜下分离110只美洲大蠊成虫肠道,用0.6%氯化钠溶液洗涤剪开的肠道,收集洗涤液,涂片并作吉氏染色,显微镜(x1000)下鉴定。结果显示,共44份肠道洗涤液涂片中发现蠊缨滴虫疑似物。虫体呈卵圆形或梨形,体长20-40μm,一端有成簇的多根鞭毛。经固定、染色后,可见虫体胞浆呈蓝色;细胞核1个,呈紫褐色,靠近虫体前端;轴柱在虫体前端呈萼状(轴罩),胞核位于其中,轴柱向后延伸至虫体末端,经鉴定确认为副基体门动鞭毛纲超鞭毛目中的蠊缨滴虫。提示武汉市美洲大蠊的蠊缨滴虫感染率为40.0%(44/110)。
An investigation of Lophomonas blattarum infection in Periplaneta americana in Wuhan City were conducted. A total of 1 l0 P. americana were dissected and the intestines were separated. The intestines were washed with 0.6% saline and the washing solutions were smeared on slides. The slides were stained with Giemsa stain and observed under a microscope (~1000). Out of ll0 intestine washing solution samples, 44 were suspected of L. blattarum infection. The parasite was oval or pyriform in shape and 20-40 [zm in size. A tuft of flagella extended clown the central axis of the parasite and a trumpet-shaped calyx enveloped the flagellar area and the nucleus. An axostyle was slender and pointed posterior ends. Based on the above morphological characteristics, the parasite was identified as L. blattarum. The results showed that the infection rate of L. blattarum in P. amerivana in Wuhan City was 40.0% (44/110).
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2014年第2期161-162,共2页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
蠊缨滴虫
美洲大蠊
感染率
Lophomonas blattarum
Periplaneta americana
Infection rate