脑星形细胞瘤基质金属蛋白酶2、9(MMPs-2,MMPs-9)的表达与瘤周水肿相关性的研究

Correlational Research on the Expression of Matrix Metalloproteinase-2, Metalloproteinase-9 of Cerebral Astrocytoma and Peritumorous Edema

目的对脑星形细胞瘤中基质金属蛋白酶2(MMPs-2)及基质金属蛋白酶9(MMPs-9)的表达水平与瘤周水肿的相关性进行探讨,为该肿瘤的临床合理诊断与治疗提供一定的科学依据。方法采用免疫组织化学染色法,在68例脑星形细胞瘤组织标本中用抗MMPs-2抗体及抗MMPs-9抗体进行标记和分析,同时采用磁共振成像技术对肿瘤瘤周水肿情况进行观察与分析,再利用数据分析软件对基质金属蛋白酶的表达量与瘤周水肿指数进行相关性分析。结果脑星形细胞瘤基质金属蛋白酶2、9的表达都随着肿瘤恶性程度的增大而增加;另外,脑星形细胞瘤基质金属蛋白酶2、9的表达水平与瘤周水肿呈现正相关的关系(p<0.05),随着MMPs-2及MMPs-9表达量的增高,瘤周水肿范围越大,且脑星形细胞瘤恶性程度越大。结论脑星形细胞瘤基质金属蛋白酶2、9的表达与瘤周水肿联系紧密,呈现正相关,这说明肿瘤分泌的MMPs-2及MMPs-9与肿瘤生长与侵袭的速度相关,这对于脑星形细胞瘤侵袭能力、恶性程度的准确预判以及治疗具有比较重要的参考意义。

Objective To investigate the correlation between the expression of matrix metalloproteinase 2 （MMPs-2） and matrix metalloproteinase 9 （MMPs-9） of cerebral asttrocyoma arid peritumotalledema, providing a scientific basis for the proper tumor diagosis and clinical treatment Methods In 68 cases of astrocytoma tissues, anti-MMPs-2 antibody and anti-MMPg-9 antibody were marked and analysis via Immunohistochemical staining, while tumor petitumoral edema were observed with analysis via magnetic resonance imaging, to make correlation analysis between the expression of matrix metalloproteinases and peritumoral ：edema index by data analysis software. Results With the：increasng,matignanc degree of tumor, the expression of matrix metalloproteinases 2,9 in atrocyt0mhwas inCreaSing, Iia addition, the expression level of astrocytoma matrix edema was positive correlation （p〈0.05）. With the ~ereasit^g of expreSsi0n level Of MMPs-2 and MMPs-9,the greater the scope of peritumoral edema and the greater mignant astrocytoma were. Condlusion Astrocytoma expression of matrix metalloproteinaSes 2.9 was close relationship with peritumoral edema, a positive correlation, the expression level of MMPs-2 and MMPs-9 was related with the rate of tumor growth and Vchich had important reference significancethe for the prediton of cerebral astrocytoma invasion, the rmalignancy degree and the treatment.