HPLC测定吴茱萸中吴茱萸碱的含量

Determination of evodiamine in evodia rutacecarpa benty by HPLC

测定吴茱萸中吴茱萸碱的含量 ,采用高效液相色谱法。色谱柱Sphevisorb -Sio2 (2 5 0mm× 4 6mm )、粒度5 μm(大连依利特公司 ) ,流动相为正庚烷 -异丙醇 -已酸 (89∶10∶1) ,检测波长 2 5 4nm ,流速 1mL·min-1。平均回收率为 10 2 5 % ,RSD =3 14%。线性关系、精密度及重线性都很好。

To determine the content of evodiamine in crude Evodia rutacecarpa Benth A HPLC method was set up.Using sphevisorb-sip 2 C 18 column(250mm×4.6mm,5 μm),n-heptane—iso-propylalconol—caproic acid (89∶10∶1) as mobile phase with a flow rate of 1.0mL·min -1 ,254nm as detection wavelength The average recovery was 102.5%,the RDS was 3.14%. It has good linearity ,precision and repetition