生物玻璃条索修复骨缺损的有效性研究

Experimental study of effects of new bioactive materials containing type Ⅰ collagen in repairing bone defect

目的评价3种新型骨移植材料的成骨效应和降解性能。方法健康成年雌性新西兰大白兔18只，采用手术方法在双侧股骨髁松质骨部建立直径6mm、深12mm的腔隙性骨缺损模型，并在胫骨近端穿刺抽取骨髓液1．5。2．0ml。将动物随机分为A、B、C3组（n=6），分别在双侧股骨髁缺损处植人自体骨髓与胶原生物玻璃条索、胶原生物玻璃颗粒、含I型胶原的B．磷酸三钙颗粒的混合物。术后6周及12周通过大体观察、X线摄片和组织学观察评估材料的成骨能力及降解性能。结果大体观察术后12周时缺损处与周围骨组织融合良好，肉眼难以区分其界线。Lane—SandhuX线评分和组织学观察显示术后6周及12周时材料成骨能力和降解性ttA组优于B、C组（P〈0．05），B组优于C组fP〈0．05）。与术后6周时比较，术后12周时3组材料的成骨量和降解量均明显增加（p〈o．01）。结论胶原生物玻璃条索不仅具有良好的生物相容性，而且成骨作用和降解性能稳定，具有良好的应用前景。

Objective To evaluate the osteogenic potential and biodegradability of three kinds of new bioactive bone graft materials. Methods Eighteen healthy adult female New Zealand white rabbits were selected. A bone defect with a hole of 6 mm in diameter and 12 mm in depth in cancellous bone of bilateral femoral condyles was established by operative method, and 1.S- 2.0ml bone marrow was harvested from the proximal tibia. Then the animals were randomly divided into three groups （n=6）, and the bone defects were filled with autologous bone marrow mixed NovaBone Collagen Bioactive Glass Strips （group A）, NovaBone Collagen Bioactive Glass Morsels （group B）, or Orthovita Vitoss Foam Pack （group C）, respectively. Specimens were collected at 6 and 12 weeks respectively after operation to evaluate the osteogenic potential and biodegradability through gross observation, X-ray radiograph and histological examination. Results Gross observation showed bony fusion of the defects with adjacent bone tissues 12 weeks after operation and the boundaries were difficult to be distinguished. Lane-Sandhu X-ray scores and histological observation showed that in terms of the osteogenic potential and biodegradability~ group A was better than groups B and C （P〈0.0S）, and group B was better than group C （P〈0.0S） 6 and 12 weeks after operation. Compared with the results as observed at 6 weeks after operation, the amounts of bone formation and material degradation were all increased 12 weeks after operation in all the 3 groups （P〈0.01）. Conclusions NovaBone Collagen Bioactive Glass Strips have favorable biocompatibility, stable osteogenic potential and biodegradability, and may be used for repairing bone defects.