肺腺癌患者胸腔积液中Axin、β-catenin和p53的表达及意义

Expression of Axin,β-catenin and p53 and their significance in pleural fluid of lung adenocarinoma patients

目的观察胸腔积液中Axin、β-catenin和p53的表达,探讨三者表达的关系及意义。方法采用免疫细胞化学SP法观察40例原发性肺腺癌患者恶性胸腔积液和40例肺良性疾病患者反应性胸腔积液中Axin、β-catenin和p53的表达。结果在原发性肺腺癌胸腔积液中,Axin阳性率为30%(12/40),β-catenin细胞膜阳性率为15%(6/40),细胞质阳性率为60%(24/40),p53阳性率为57.5%(23/40)。Axin阳性率与β-catenin细胞膜表达呈正相关,与p53表达呈负相关。在肺良性疾病胸腔积液中Axin阳性率为77.5%(31/40),β-catenin无阳性表达,p53阳性率为1.25%(5/40)。结论在原发性肺腺癌胸腔积液中Axin表达降低,β-catenin细胞质阳性率高于细胞膜,差异有显著性(P<0.05),p53呈过表达。提示恶性胸腔积液中的癌细胞可能存在Wnt信号的激活。

Purpose To investigate the expression of Axin, β-catenin and p53 in the pleural fluid and to discuss their relationships and significances. Methods The expression of Axin, β-catenin and p53 was detected using immunocytochemistry in adenocarinoma cells of the pleural fluid from 40 patients with primary lung adenocarcinomas or reactive mesothelial cells of the pleural fluid from 40 pa- tients with benign lung diseases. Results Axin positive expression rate was 30% （12/40） in cases with primary lung adenocarcino- mas. The positive expression rate of β-catenin was 15% （6/40） on cell membrane and 60% （24/40） in cytoplasm. The expression rate of p53 was 57.5% （23/40） in cases of primary lung adenocarcinomas. The expression of Axin was positively correlated with the membranous expression of β-catenin, and negatively correlated with p53 in cancer cells of the pleural fluids. The positive expression rate of Axin was 77.5% （31/40）, but the expression rate of p53 was 1.25% （5/40）. There was no expression of β-catenin in reac- tive mesothelial ceils from benign lung diseases. Conclusions The expression of Axin is significantly reduced in adenocarcinoma cells. The expression rate of β-catenin in cytoplasm is higher than that in membrane of cancer cells. It has a certain value to detect the expression of Axin, β-catenin and p53 for diagnosis and differential diagnosis of lung adenocaicinomas from benign lung diseases.