早期喉癌手术阴性切缘组织中PCNA、COX-2表达与肿瘤局部复发的关系

Relationship between expression of PCNA and COX-2 and local recurrence of tumor in early laryngeal cancer with negative surgical margins

目的探讨早期喉癌手术阴性切缘组织中PCNA、COX-2的表达与肿瘤局部复发的关系。方法选择63例经CO2激光手术治疗后的早期喉癌患者标本,对切除的肿瘤组织及切缘组织中PCNA、COX-2表达进行检测,所有患者均随访2年以上,对其生存及肿瘤复发情况进行观察。结果 63例早期喉癌患者肿瘤组织中PCNA、COX-2阳性率分别为73.02%、71.43%;切缘组织中PCNA和COX-2阳性率分别为33.33%、30.16%。肿瘤组织与切缘组织中PCNA、COX-2均阳性的患者分别为41例和13例,阳性率分别为65.08%、20.63%;63例随访病例中,局部复发17例,复发率为26.98%。PCNA阳性患者复发率为71.43%,COX-2阳性患者复发率为73.68%(P<0.05)。联合检测PCNA和COX-2阳性者复发率明显高于单独阳性者(P<0.05)。结论早期喉癌手术切缘组织中PCNA、COX-2的表达是喉癌患者预后的重要生物学标志,并可根据检查结果制定针对性的后续治疗方案,联合检测二者对术后治疗具有更可靠的指导意义。

Purpose To explore the relationship between expression of PCNA and COX-2 in the early laryngeal cancer with negative surgical margins and the local recurrence of tumor. Methods Totally 63 patients with early laryngeal cancer were enrolled in this stud- y, CO2 laser surgery was adopted as treatment, the expression of PCNA and COX-2 was detected in the resected tumor tissue and surgi- cal margins, and the survival and tumor recurrence were also observed. Results The positive rate of the expression of PCNA and COX-2 in the 63 patients with early laryngeal cancer tumor tissues were 73.02% and 71.43% , while that in the cutting edge were 33. 33% and 30.16% , respectively. The expression of PCNA and COX-2 in tumor tissue and cutting edge were of all positive in 41 cases and 13 cases, and the positive rate was 65.08% and 20. 63%, respectively. In the all followed-up patients, 17 cases were detected of local recurrence, the recurrence rate was 26. 98%, and the recurrence rate in the patients with PCNA positive was 71.43%, while in the patients with COX-2 positive was 73.68% （ P 〈 0. 05 ）. The combined detection of PCNA and COX-2 positive recurrence rate was significantly higher than that of single positive （ P 〈 0. 05 ）. Conclusion PCNA and COX-2 in the early laryngeal cancer with negative surgical margins are important biological markers for the evaluation of prognosis of laryngeal cancer patients, and to formulate the subse- quent treatment regimen. The combined detection of two proteins is more reliable for postoperative management.