青春期暴露氰戊菊酯对小鼠海马雄激素受体和雌激素受体的影响

Effects of pubertal fenvalerate exposure on androgen receptor and estrogen receptors in hippocampus of mice

目的探讨青春期小鼠暴露氰戊菊酯（Fen）对其大脑海马的雄激素受体（AR）和雌激素受体n（ERα）、雌激素受体B（ERβ）蛋白表达水平的影响。方法选取60只3周龄ICR小鼠，随机均分为空白组、溶剂对照组、Fen（0．02、0．2、2mg／kg）处理组，每组雌雄各半，在出生后28～56d里，Fen处理组给予相应量的Fen，溶剂对照组给予等容积的玉米油，空白组不予任何处理。灌胃4周后处死小鼠，取其大脑海马组织，采用Western blot法测定海马AR、ERα和ERβ的表达水平。结果①青春期暴露Fen引起雄鼠海马AR表达明显升高，雌鼠海马AR表达未发生显著性改变；②青春期暴露Fen雄鼠海马ERα和ERβ表达组间差异无统计学意义，雌鼠海马ERα和ERβ表达亦无显著变化。结论青春期暴露Fen干扰雄性小鼠海马AR的表达。

Objective To investigate the effects of pubertal fenvalerate exposure on the expression of androgen re- ceptor （AR） and estrogen receptors （ERs） in hippocampus. Methods 60 male and female mice in postnatal day （PND） 28 were randomly divided into five groups： the blank group, the control group and fenvalerate （0. 02, 0. 2, 2 mg/kg） group. Pubertal male and female mice in treatment groups were treated with fenvalerate （ 0. 02, 0. 2, 2 mg/kg） by gavage daily from PND28 to PND56, while the blank gave nothing and controls received corn oil. After four weeks, the mice were sacrificed, then hippocampus was excised. Protein expression of AR, two estro- gen receptors（ ERα and ERβ） in hippocampus were analyzed by Western blot. Results In males, the protein ex- pression of AR in hippocampus was markedly upregulated in pubertal fenvalerate exposure. In females, the protein expression of AR in hippocampus was of no statistically significant differences between groups. There was no statis- tically significant difference of the ERα and ERβ protein expression among all the sexual groups. Conclusion Pu- bertal fenvalerate exposure disrupts AR expression in hippocampus of mice.