CXCL16及CXCL10在狼疮性肾炎患者中的表达及与疾病活动的相关性

Expression of CXCL16 and CXCL10 correlates with disease activity in lupus nephritis patients

目的探讨趋化因子配体16(CXCL16)及趋化因子配体10(CXCL10)在狼疮性肾炎(LN)患者血清、尿液以及肾脏内的表达以及与疾病活动的关系。方法 ELISA法检测LN患者及正常对照者血清、尿液中CXCL16、CXCL10的表达水平,免疫组化法检测经肾脏活检确诊为LN的患者以及正常对照者肾脏组织中的CXCL16的表达水平。结果 LN患者血清以及尿液中的CXCL16、CXCL10与正常对照者比较均升高(P<0.01),且血清中CXCL10水平与系统性红斑狼疮疾病活动指数(SLEDAI)评分呈正相关(r=0.39,P<0.01),与补体C3呈负相关(r=-0.49,P<0.01),尿液中CXCL16水平与SLEDAI评分呈正相关(r=0.34,P<0.05)。正常对照组肾脏的小管上皮细胞可见CXCL16较强的表达,肾小球很少表达,但在LN患者可见小管中表达有所增强,小球中明显增强,其肾内的CXCL16的表达水平较正常对照组上升(P<0.05),尤以Ⅳ型显著(P<0.05)。结论 CXCL10、CXCL16可能参与LN的发病过程,血清CXCL10和尿液中CXCL16水平的检测对于判断疾病的临床活动具有一定的意义,其中CXCL16可能与LN的病理变化有关。

Objective To discuss the expression of CXC Chemokine Ligand 16 （CXCL16） and CXC Chemokine Ligand 10 （ CXCL10） in serum, urine and renal tissue in lupus nephritis （LN）. Methods The serum and urine levels of CXCL10, CXCL16 were assessed by ELISA in LN patients and normal controls. Immunohistochemistry was used to detect the renal tissue expressions of CXCLl6 in patients and healthy controls. Results The serum and urine concentrations of CXCL10 and CXCL16 were significantly higher in LN patients than those in healthy controls （p 〈 0.01）. In addition, the level of serum CXCL10 showed positive correlation with SLEDAI and negative correlation with serum C3 level （ P 〈 0.01） ,the expression of urine CXCL16 showed positive correlation with SLEDAI （P 〈 0. 05） . In healthy renal tissues CXCL16 was distributed mainly in renal tubules and rarely seen in glomerular cells while the expressions in renal tubules increased and glomeruli stainings were found in LN paitients. CXCL16 expressed significantly in LN patients than normal controls （P 〈 0. 05） . Besides, different concentrations of CXCL16 were observed in ill, N and V LN, the expressions of CXCL16 in N LN were higher than those in ill LN and V LN （P 〈 0.01） ,while no difference between the ill LN and V LN. Conclusion CXCL10 and CXCL16 may play key roles in the pathogenesis of LN and both of CXCL10 in serum and CXCL16 in urine may be useful as markers of disease activity. The change of CXCL16 may associate with the pathology category of LN.