溃疡性结肠炎患者外周血中MMP-2、MMP-9与肠黏膜通透性关系的临床研究

The relationship between the expression of matrix metalloproteinases and intestinal mucosal permeability in patients with ulcerative colitis

目的探讨溃疡性结肠炎(UC)患者中基质金属蛋白酶(MMP)-2、MMP-9水平与肠黏膜通透性的临床关联,为UC的发病机制及治疗提供可靠的参考指标。方法采用ELISA法检测38例UC患者(UC组)和30例正常对照者(正常对照组)外周血中MMP-2、MMP-9水平;采用高效液相色谱示差法检测UC组和正常对照组口服乳果糖(L)和甘露醇(M)测试液后尿液中L、M含量,计算L/M比值。结果 UC组外周血中MMP-2、MMP-9水平显著高于正常对照组(P<0.05)。UC组外周血中MMP-2、MMP-9水平与UC疾病严重程度及病变范围呈一定相关性;UC组患者尿中L/M比值显著高于正常对照组(P<0.05),UC患者外周血中MMP-2、MMP-9水平与肠黏膜通透性改变有良好的相关性。结论活动期UC患者肠黏膜通透性增高,可能与外周血中MMP-2、MMP-9水平增高有关。

Objective To investigate the relationship between the expression of matrix metalloproteinases （ MMPs） and intestinal mucosal permeability in patients with ulcerative colitis. Methods 38 UC patients and 30 healthy controls were enrolled in this study, the levels of MMP-2 and MMP-9 in the serum were assessed by ELISA method. Detected the lactulose and mannitol in urine of 38 UC patients and 30 healthy people after they took orally the lactulose and mannitol test solution by HPLC-RI method and calculated the ratio of them. Results The serum levels of MMP-2 and MMP-9 in UC patients were significantly higher than that in healthy controls, which were correlated with the severity and extent of disease （ P 〈 0. 05 ）. The lactulosel mannitol ratio in 38 U C patients was signifi- cantly higher than that in 30 control people. The serum levels of MMP-2 and MMP-9 in UC patients were correlated with the the change of intestinal mucosal permeability. Conclusion The serum levels of MMP-2 and MMP-9 in UC patients are significantly increased, which is correlated with the change of intestinal mucosal permeability.