摘要
目的探讨凋亡抑制蛋白(IAP)Livin和半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)在口腔鳞状细胞癌(OSCC)中的表达及其相关性,为对患者诊断及监测、评估术后复发、转移的风险程度提供参考。方法采用RT-PCR法对45例OSCC组织及11例正常口腔组织(NOT)中的Livin mRNA与Caspase-3 mRNA的表达进行检测。结果 Livin mRNA与Caspase-3 mRNA在45例OSCC组织及11例NOT组织均有不同程度的表达;Livin mRNA在OSCC组织中的表达高于NOT组织(P<0.05),在有淋巴结转移组的表达高于无淋巴结转移组,差异有统计学意义(P<0.05)。两者在OSCC组织中的相对含量均与患者的性别和年龄无关,但与肿瘤的病理分期、分化程度有关,相关分析显示Livin mRNA与Caspase-3 mRNA在OSCC组织中的表达呈相关关系,差异有统计学意义(P<0.01)。结论 Livin与Caspase-3基因的异常表达与OSCC的病理生物学行为密切相关,联合检测Livin与Caspase-3可为评价OSCC的浸润、转移及患者预后提供临床参考,为研究OSCC发病机制提供新的思路。
Objective To investigate the expression and relativity of Livin and Caspase-3 in oral squamous cell carcinoma （ OSCC）. Methods The expression of Livin mRNA and Caspase-3 mRNA in 45 cases of OSCC and 11 cases of normal oral tissue were detected by reverse RT-PCR. Results The positive expression rates of Livin mRNA in OSCC were significantly higher than those in normal oral tissue （P 〈 0. 05）. Compared with those without lymph node metastasis in OSCC, the positive ratios of Livin mRNA with lymph node metastasis were significantly higher（ P 〈 0. 05） . The expressions of Livin were significantly related with pathological stage and the lymph node metastasis, but neither of them was correlated with sex and age. Correlation analysis showed that Livin expression level was related to Caspase-3 level （P 〈 0. 01 ）. Conclusion The abnormal expressions of Livin and Caspase-3 are closely related to the occurrence, progress and metastasis of OSCC , which can evaluate the degree of malignancy and prognosis of patients with OSCC reference.
出处
《安徽医科大学学报》
CAS
北大核心
2014年第4期498-501,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省卫生厅科研项目(编号:2010-B-15)
