摘要
25株临床分离的热带念珠菌通过ATB FUNGUS 3试条分类为对氟康唑(FCA)敏感的菌株和耐药的菌株,使用ABI 7300荧光定量分析仪扩增外排基因CDR1、MDR1和靶酶基因ERG11,用2-ΔΔCT法计算相对表达量,应用Fisher确切概率法统计分析敏感菌株和耐药菌株的高表达率。结果显示MDR1和ERG11基因的高表达率差异有统计学意义(P<0.05),CDR1基因的高表达率差异无统计学意义(P>0.05)。热带念珠菌对FCA耐药与外排基因MDR1和靶酶基因ERG11的高表达相关。
Fluconazole（FCA）-susceptible and resistant isolates were classified by ATB FUNGUS 3 strip from 25 clinical Candida tropicalis, efflux gene CDR1, MDR1 and enzyme gene ERG11 were amplified by ABI 7300 Taq- Man analyzer, relative expression quantities were calculated by 2^-△△CT method, overexpression rates of sensitive iso- lates and resistant isolates were statistically analyzed using Fisher exact probability method. There were statistically significant differences in the overexpression rates of MDR1 and ERG11 （ P 〈 0.05 ） , no statistically significant difference in the overexpression rate of CDR1 （P 〉 0. 05）. Candida tropicalis resistance to FCA was mediated by overexpression of efflux gene MDR1 and enzyme gene ERG11.
出处
《安徽医科大学学报》
CAS
北大核心
2014年第4期536-539,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省教育厅基金(编号:KJ2008B299)
