CD4+CD25+Treg调节性T细胞及其细胞因子在大鼠动脉粥样硬化中改变的研究

Study on the changes of CD4+ CD25+ Treg regulatory T cells and their cytokine secretion in rats with atherosclerotic

目的研究CD4+CD25+Treg调节性T细胞及其分泌的细胞因子在大鼠动脉粥样硬化中的改变。方法制备动脉粥样硬化模型,取雄性SD大鼠30只,随机分为正常饮食组(A组)10只及高脂饮食组(B组)20只,高脂饮食组再分为对照组与干预组各10只,干预组高脂饲料喂养前腹腔注射抗CD25+单克隆抗体250μg,A组及对照组腹腔注射大鼠IgG抗体(250μg),高脂饮食组定期用维生素D腹腔注射。上述各组喂养3个月后,各组随机抽取6只大鼠通过流式细胞学技术检测CD4+CD25+Treg细胞及Foxp3+的表达、检测炎症因子IL-10,IL-1β水平,处死各组大鼠做病理切片。结果 3组中干预组CD4+CD25+Treg细胞及Foxp3+表达下降最明显,IL-10水平明显下降,IL-1β水平明显升高,A组CD4+CD25+Treg细胞及Foxp3+表达最高,IL-10水平亦最高,IL-1β水平最低。而对照组在干预组及A组之间。结论动脉粥样硬化形成中CD4+CD25+Treg调节性T细胞及其分泌的细胞因子受到抑制,而不是CD4+CD25+Treg调节性T细胞分泌的因子却显著升高,考虑动脉粥样硬化的形成有免疫反应参与。

[ Abstract ] Objective To research the change of CD4 ＋ CD25 ＋ Treg regulatory T cells and their cytokine secretion in rats with atheroselerosis. Methods The models of atherosclerosis was made. Male SD rats were randomly divided into normal diet group （A 10 cases） and high-fat diet group （B 20 eases） ,the high fat diet group was further divided into a control group and intervention group each of 10 cases, the intervention group before high-fat diet were injected anti-CD25 ＋ monoclonal anti- body 250μg. The control group and group A were injected IgG antibody （250ug） of rat, the high fat diet group regular use of vitamin D by intraperitoneal injection. The above-mentioned groups were fed for three months, Each group were randomly se- lected six rats via flow cytometry to detect CD4 ＋ CD25 ＋ Treg cells and Foxp3 ＋ expression, and detected the levels of inflam- matory cytokines IL-10,IL-1β,also the rats of each group were sacrificed do biopsy. Results The CD4 ＋ CD25 ＋ Treg cells and Foxp3 ＋ expression of intervention group decreased most significantly in the three groups, the levels of IL-10 were also sig- nificantly decreased, and the levels of IL-113 were significantly increased. The CD4 ＋ CD25 ＋ Treg cells and Foxp3 ＋ expression of group A were the highest among the three groups, IL-10 levels were the highest too, IL-1βlevel was the lowest. While the control group was in between the intervention group and group A. Conclusion CD4 ＋ CD25 ＋ Treg regulatory T cells and their cytokine secretion in atherosclerosis was inhibited, rather than CD4 ＋ CD25 ＋ Treg regulatory T ceils secreted factor was signifi- cantly elevated. The formation of atherosclerosis is involved in immune responses.