米非司酮调控乳腺癌细胞增殖的研究

Effect of mifepristone on the proliferation of human breast cancer cell line MCF-7and T47D in vitro

目的研究米非司酮对乳腺癌细胞系MCF-7和T47D的增殖抑制作用。方法采用四甲基偶氮唑盐(MTT)法检测米非司酮对乳腺癌细胞系MCF-7和T47D的半数抑制浓度(IC50)。不同浓度米非司酮(0.25、2.5、25和50μmol/L)分别处理人乳腺癌细胞系MCF-7和T47D1、2、3d后,观察细胞形态变化,以及检测各组细胞生长曲线。结果米非司酮对乳腺癌MCF-7和T47D细胞均有抑制作用,其抑制率均随着米非司酮浓度的增加而变化,存在明显的量效关系。通过对两种细胞抑制率(IR)值直线回归分析,米非司酮对MCF-7细胞的IC50为51.21μmol/L,对T47D细胞的IC50为54.29μmol/L。形态学观察发现,各组细胞体积变小,细胞间的链接消失,细胞内明显有黑色小颗粒聚集。25和50μmol/L浓度米非司酮对MCF-7乳腺癌细胞株生长抑制作用明显,特别是50μmol/L干预24h后在高倍镜下可见明显的核质浓缩。结论米非司酮对病理分型、激素受体型不同的乳腺癌MCF-7和T47D细胞均具有抑制增殖的作用,并呈时间和剂量依赖效应。

Objective. To explore the effect of mifepristone on proliferation of human breast cancer cells MCF-7 and T47D in vitro. Methods. MCF-7 and T47D cells were treated by different concentrations（0. 25,2.5,25 and 50 μmol/L）of mifepristone. Then the growth inhibitions of mifepristone were examined by MTT assay on day 1,2,3 to median effective inhibitory concentration 50% （IC50）and growth curves. The cell morphology changes were also observed. Results： Mifepristone had inhibiting effects on both MCF-7 and T47D cells,of which the inhibiting effects were dependent on the concentration. The IC50 was 51.21 μmol/L in MCF-7 cells and 54.29 /lmol/L in T47D cells respectively,which was revealed by linear regression of IR values. Cell morphology observation found that cell volume became smaller, cell-cell connection disappeared and small black granules were clustered in both cells. The inhibiting effects in high concentrations（25 and, 50 μmol/L）were stronger than those of low concentrations （0.25 and 2.5 μmol/L）on both cancer cells. Of note, clear concentrated karyoplasms were observed 24 hours after 50μmol/L mifepristone treatment in both cells. Conclusions： Mifepristone inhibits the proliferation of both MCF-7 and T47D cells in a dose-dependent manner.