多西紫杉醇用于肿瘤热化疗的体外研究

In vitro investigation on the application of docetaxelin cancer thermochemotherpy

目的:研究不同剂量的多烯紫杉醇(docetaxel,DTX)联合不同温度的热疗对两种肝癌细胞(HepG2、BEL-7402)的体外毒性,探讨热疗对化疗是否具有协同增敏效应,为肝肿瘤的热化疗提供理论依据。方法:CCK-8法测定不同浓度DTX对细胞的毒性作用,SPSS 17.0软件分析DTX作用于两种细胞的IC50值,并进一步研究温度对两种细胞IC50值的影响,Veleriote法分析该药物浓度下的热化疗是否存在协同增敏效应。结果:DTX对HepG2和BEL-7402的IC50值分别为0.152μg/ml和0.563μg/ml。热疗可有效抑制两种细胞的生存率,热化疗联合处理可显著降低两种细胞的存活率,且该综合治疗方法在一定温度下存在协同增敏效应。结论:HepG2和BEL-7402两种细胞对DTX均很敏感,其IC50值的不同提示了临床上个性化治疗的重要性。热疗联合DTX的综合治疗明显优于单一治疗,热化疗的协同增敏效应呈温度依赖性。

Objective：To investigate the in vitro cytotoxicity of docetaxel （DTX）combined with hyperthermia on hepatoma cells （HepG2, BEL -7402）. Methods： CCK -8 assay was applied to evaluate the cell viability and the ICs0 values were determined by SPSS 17. O. Effect of heating treatment with different temperatures on the ICs0 values of HepG2 and BEL -7402 cells was studied. Velefiote method was applied to analyze the synergistic effect betweendocetaxel and heating treatment on the two cell lines. Results ： The ICs0 values of DTX on HepG2 and Be1 - 7402 cells were determined to be 0. 152~g/ml and 0. 563 ~g/ml, respectively. While hyperthermia effectively inhibited the via- bility of the two hepatoma cells, DTX combined with heating treatment could lead to a even greater decrease on the cell viability, as compared with either drug or heating treatment only. There may exist synergistic effect between DTX and heating treatment and such effect is temperature dependent. Conclusion：Both hepatoma cells involved in the cur- rent research（ HepG2 and BEL -7402）are sensitive to the drug treatment by DTX. The different of ICs0 values of DTX on the two cell lines suggests the significance of personalized treatment for cancer. The bi - modal treatment by DTX combined with heating is more effective then mono - treatment. Synergistic effect between heating treatment and DTX is temperature dependent.