LSD1、E-钙黏蛋白、N-钙黏蛋白在结肠癌中的表达及其意义

Expressions of LSD1,E-cadherin and N-cadherin in colon cancer and their significance

目的:探讨赖氨酸特异性组蛋白去甲基化酶1(LSD1)、E-钙黏蛋白(E-cad)、N-钙黏蛋白(N-cad)在结肠癌组织中的表达及其临床意义。方法:收集2006年2月—2008年12月于中南大学湘雅医院行手术切除的结肠癌标本108例,采用免疫组化技术检测结肠癌组织中LSD1、E-cad、N-cad的表达,并分析其与患者临床病理特征及预后的关系。结果:全组LSD1、E-cad、N-cad的阳性表率达分别为66.7%(72/108),85.2%例(92/108)、41.7%(45/108)。LSD1在较晚TNM分期、远处转移的结肠癌组织中的表达率明显增高,而E-cad则相反(均P<0.05),N-cad的表达在各项临床病理因素分组间差异均无统计学意义(均P>0.05)。LSD1与E-cad在结肠癌组织中的表达呈明显负相关(r=-0.318,P=0.001),与N-cad的表达无明显相关性(r=0.182,P=0.06)。LSD1的阳性表达组和E-cad的阴性表达组患者总生存率明显低于各自的阴性表达组与阳性表达组(均P<0.05),N-cad表达与否与患者总生存率无明显关系(P=0.410)。结论:LSD1和E-cad的表达与结肠癌的转移有密切关系,阳性表达LSD1和阴性表达E-cad的结肠癌预示预后不良。

Objective： To investigate the expressions of lysine-specific demethylase 1 （LSD 1）, E-cadherin （E-cad） and N-cadherin （N-cad） in colon cancer and their clinical significance. Methods： Cancer tissue specimens from 108 cases of colon cancer who underwent operation at Xiangya hospital from February 2006 to December 2008 were collected, q-he expressions of LSDI, E-cad and N-cad in these colon cancer tissues were determined by immunohistochemical staining, and their relations with clinicopathologic characteristics and prognosis of the patients were analyzed. Results： In the entire group, the positive expression rate of LSD1, E-cad and N-cad in colon was 66.7% （72/108）, 85.2% （92/108） and 41.7% （45/108）, respectively. Positive LSD1 expression rate was significantly increased in tumors with advanced TNM stage and distant metastasis, which for E-cad was the opposite （all P〈0.05）, while there were no statistical differences in N-cad expression among the groups divided according to clinicopathologic factors （all P〉0.05）. In colon cancer tissues, the LSD1 expression was negatively correlated with E-cad expression （r=-0.318, P=0.001）, but showed no significant correlation with N-cad expression （r=0.182, P=0.06）. The overall survival rate in LSD 1 positive expression group and E-cad negative expression group was significantly lower than that in their respective negative expression group and positive expression group （both P〈O.05）, while positive or negative N-cad expression yielded no significant relation with the overall survival of the patients （P=0.410）.