Pleiotrophin的表达及其在血清水平与结肠癌的关系

Relation of pleiotrophin expression and its serum level with colorectal cancer

目的:探讨pleiotrophin(PTN)的表达及其血清水平与结肠癌的关系。方法:应用real time-PCR、Western blot方法检测46例结直肠癌组织及其对应癌旁组织中PTN mRNA及蛋白表达水平;ELISA方法检测76例结直肠癌患者和58例健康体检者血液样本中的PTN表达水平。结果:PTN mRNA在结直肠癌组织中表达量以及PTN蛋白的阳性表达率均明显高于癌旁正常组织,差异均有统计学意义(均P<0.05);无论是PTN mRNA表达水平还是蛋白阳性率均与患者年龄、肿瘤组织类型无关(均P>0.05),而与肿瘤的分化程度呈负向关系、与TNM分期呈正向关系(均P<0.05)。结直肠癌患者血清PTN水平明显高于正常人群(P<0.05)。结论:PTN在结直肠癌组织中表达增高;血清PTN水平检测可作为诊断结直肠癌参考指标之一。

Objective： To investigate the relations of pleiotrophin （PTN） expression and its serum level with colorectal carlcer. Methods： l-he PTN mRNA and protein expression in tumor tissue specimens of 46 cases of colorectal cancer and in their adjacent tissue was determined by real-time PCR and Western blot, respectivel and the PTN level in the blood samples from 76 patients with colorectal cancer and 58 subjects undergoing health maintenance examination was measured by ELISA assa~ Results： Both the PTN mRNA expression level and PTN protein positive expression rate in colorectal cancer tissue were significantly higher than those in the adjacent normal tissue （both P〈0.05）, and either the PTN mRNA expression level or PTN positive rate was not associated with age of the patients or histological type of the tumor （all P〉0.05）, but showed a significantly negative relation with TNM classification （all P〈0.05）.The serum PTN level in colorectal cancer patients was significantly higher than that in normal subjects （P〈0.05）. Conclusion： PTN expression is increased in colorectal cancer tissue and serum PTN level may be used as one ofthe diagnostic tests for colorectal cancer.