白细胞介素16基因多态性与大肠癌发病风险的关系

Relationship between interleukin 16 polymorphism and risk of colorectal cancer

目的:探讨中国山东籍汉族大肠癌(CRC)患者人群中,IL-16基因多态性与大肠癌发病风险的关系。方法:在164例CRC患者和121名健康对照个体中,采用放大阻碍突变PCR(ARMS-PCR)方法对IL-16基因序列中的2个单核苷酸多态性(SNP)位点rs11556218和rs4778889进行基因分型,分析该2个位点的等位基因频率和基因型分布与CRC发病风险的关系。结果:在CRC患者中,rs11556218的T/G等位基因频率和基因型分布与对照组的差异有统计学意义(P=0.021 725,P=0.001 033);rs4778889的T/C等位基因频率及基因型分布与正常对照组的差异无统计学意义(P=0.057 946,P=0.064 229)。结论:IL-16的rs11556218基因多态性与可能与CRC的易感性增加有关。

Objective： To investigate the relationship between interleukin 16 （IL-16） gene polymorphism and risk of colorectal cancer （CRC） in Han nationality population in Chinas Shandong province. Methods： From 164 CRC patients and 121 control healthy subjects, two single nucleotide polymorphism （SNP） sites, rs 11556218 and rs4778889, in the IL-16 gene sequence were genotyped by means of amplification refractory mutation system （ARMS）-PCR. The relations of the allele frequency and distribution of the two polymorphic sites with CRC risk were analyzed. Results： In group of CRC patients, both the T/G allele frequency and distribution ofrs11556218 were significantly different from control group （P=0.021 725, P=0.001 033）, but the differences in the T/C allele frequency and distribution ofrs4778889 had no statistical significance versus control group （P=0.057 946, P=0.064 229） Conclusion： IL- 16 rs 11556218 gene polymorphism is probably associated with the increased CRC susceptibility.