实验性应激对糖尿病大鼠血糖和内分泌激素的影响及干预

Effects of experimental stress on serum glucose and endocrine hormone in diabetic rats and its intervention with insulin andfluoxetine

目的探讨实验性应激对糖尿病大鼠血糖、胰岛素、胰高糖素、皮质醇、肾上腺素水平和胰岛素抵抗指数的影响以及胰岛素和氟西汀对此的干预作用。方法实验大鼠分为正常大鼠对照组、糖尿病大鼠对照组、糖尿病大鼠胰岛素组、糖尿病大鼠氟西汀组，高糖高脂饲养加腹腔少量注射链脲菌素诱导糖尿病大鼠模型，各组大鼠进行为期8周的随机强迫游泳、寒冷、旋转、限制、拥挤等实验性应激，实验第4、8周分别采血测定血清血糖、胰岛素、胰高糖素、皮质醇、肾上腺素水平。结果糖尿病大鼠应激后血糖[（16．7±3．5）mmol／LVS（5．1±1．1）mmol／L，t=13．9，P〈0．01]、胰高糖素[（158．5±50．2）ng／LVS（120．8±38．7）ng／L，t=2．5，P〈0．05]、肾上腺素[（203．8±48．6）pg／mlVS（158．7±42．6）pg／ml，t=2．9，P〈0．01]、皮质醇水平[（21．3±4．8）ng／mlvS（18．2±3．8）ng／ml，t=2．1，P〈0．05]及胰岛素抵抗指数（10．9±2．6VS3．3±0．8，t=12．3，P〈0．01）明显升高，胰岛素治疗后血糖[（9．7±2．1）mmol／LVS（16．7±3．5）mmolfL，t=7．0，P〈0．01]明显改善，氟西汀治疗后肾上腺素[（158．8±37．5）pg／mlvS（203．8±48．6）pg／ml，t=3．0，P〈0．01]、皮质醇[（15．7±4．2）ng/mlvS（21．3±4．8）ng／ml，t=3．5，P〈0．01]明显改善。结论实验性应激导致糖尿病大鼠血糖和内分泌激素升高，加重胰岛素抵抗，胰岛素能改善应激后血糖变化，氟西汀能改善应激后内分泌激素的升高。

Objective To investigate the impact of experimental stress on serum glucose, insulin, glucagon, cortisol, and ep- inephrine in diabetic rats and its intervention with insulin and fluoxetine. Methods Type 2 diabetic rats model was induced by feeding with high sugar and high fat diet, and intraperitoneal injection of streptozotocin （STZ）. The rats were divided into normal group, dia- betic control group, diabetic treated with insulin, and diabetic treated with fluoxetine. All the rats were exposed to multiple stressors （forced swimming, cold stimulation, rotation, restrain, and crowding） for 8 weeks. The rat blood sera collected from each group were analyzed with multiple parameters including glucose, insulin, glucagon, cortisol, and epinephrine at the 4th and 8th week. Results After experimental stress, the levels of glucose [ （ 16. 7 ± 3.5 ） mmol/L vs （5.1 ± 1. 1 ） mmol/L, t = 13. 9, P 〈 0. 01 ], glucagon [ （ 158.5 ± 50. 2 ） ng/L vs （ 120. 8 ± 38. 7 ） ng/L, t = 2. 5, P 〈 0. 05 ], epinephrine [ （ 203.8 ± 48. 6 ） pg/ml vs （ 158.7 ± 42. 6 ） pg/ ml, t = 2. 9, P 〈 0. 01 ], cortisol [ （21.3 ± 4. 8） ng/ml vs （ 18. 2 ~ 3.8） ng/ml, t = 2. 1, P 〈 0. 05 ], and HOMA-IR （ 10.9 ± 2.6 vs 3.3 s0. 8, t = 12. 3, P 〈0. 01 ） of diabetic rats were significantly increased. The glucose level [ （9. 7 ±2. 1 ） mmol/L vs （ 16. 7 ± 3. 5） mmol/L, t = 7.0, P 〈 0. 01 ] was significantly improved after treated with insulin. The levels of epinephrine [ （ 158.8 ± 37. 5 ） pg/mlvs （203.8±48.6）pg/ml, t =3.0, P 〈0.01], and cortisol [（15.7±4.2）ng/ml vs （21.3±4.8）ng/ml, t =3.5, P 〈 0. 01 ] were signifieanfly improved after treated with fluoxetine. Conclusions Experimental stress increased the levels of glucose, en- docrine hormone, and insulin resistance of diabetic rats. Treatment with insulin improved the glucose level and treatment of fluoxetine improved the endocrine hormone level.