紫甘薯花青素对高脂饮食导致小鼠脑部炎症反应的保护作用

Protective Effect of Purple Sweet Potato Color On the Neuroinflammation Induced by High Fat Diet in T2DM Mice

目的本研究利用HFD(high fat diet)建立肥胖小鼠神经炎症模型,探讨紫甘薯花青素(purple sweet potato color,PSPC)对HFD诱导的肥胖小鼠脑部神经炎症损伤的影响及分子机制。方法 ICR(institute of cancer research)雄性小鼠100只,按随机数字表法随机分为4组,每组25只,分别为:正常对照组、HFD组、HFD+PSPC组、PSPC组。HFD+PSPC组、PSPC组灌喂PSPC 700mg/(kg·d),正常对照组、HFD组灌喂同等体积的0.9%的生理盐水,连续喂养灌喂20周,每周两次称量小鼠空腹体重。于第20周进行行为学检测,行为学检测之后立即断颈处死,取脑,分别检测海马与前脑皮质中的COX-2(cyclo-oxygenase-2)在不同处理组的小鼠脑组织中的蛋白表达水平。结果 HFD处理的小鼠体重有显著的升高,而PSPC能够有效改善上述症状,显著改善HFD诱导的小鼠自发活动能力和学习记忆能力的下降,显著抑制HFD诱导的小鼠海马和前脑皮质COX-2的蛋白表达水平增加。结论研究表明,紫甘薯花青素具有神经保护作用,可能的机制是抑制炎症相关蛋白的表达,从而起到抗炎作用。

Objective To investigate the effect of PSPC on the neuroflammation in HFD-induced mouse brain and discuss the mechanism of the protective effect.Methods Male ICR mice were randomly divided into 4 groups,termed as group control,group HFD,group HFD ＋ PSPC,and group PSPC.Mice in group PSPC and group HFD ＋ PSPC were lavaged with purple sweet potato color in 0.9％ saline containing 0.1％ Tween 80 at a dose of 700mg/（kg · d） for 20 weeks,at the same time,mice in group control and group HFD severed as control group were given saline （0.9％） orally at the same dose.The mice weight was recorded twice a week.In 20th week,the mice were used to behavior tests and protein assays.Results Mice in HFD group had significant increase in the body weight and glucose level,compared with the control group.PSPC could significantly reverse the impairment of motor and exploration behavior induced by HFD in the open field tasks,and also improve learning and memory ability in step-through tests,could markedly decrease the high level expression of COX-2 in hippocampus and frontal cortex of brain that treated with HFD.Conclusion The results above showed that PSPC had anti-inflammatory property.The possible mechanism is that PSPC decreases the expression of inflammatory protein COX-2.