哮喘小鼠肺泡灌洗液中白细胞介素-17含量变化

Changes of Interleukin- 17 Content in Bronchoalveolar Lavage Fluid of Asthmatic Mice

目的探讨哮喘小鼠肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中白细胞介素-17(IL-17)含量的变化。方法将64只BALB/c小鼠分为正常组32只,哮喘组32只,两组分别于造模后第3、7、14、21天取小鼠肺泡灌洗液,每次8只。应用HE染色法观察哮喘组及正常组各时间点小鼠肺组织情况。应用ELISA法检测小鼠BALF中IL-17的含量。结果哮喘组的肺组织炎症反应较同期正常小鼠明显,哮喘组较正常组BALF中IL-17含量有显著升高,差异有统计学意义(P<0.05)。哮喘组第7天小鼠BALF中IL-17含量达到高峰,同期小鼠肺组织HE染色炎症亦最明显。结论哮喘组小鼠BALF中IL-17含量升高,且在造模后第7天达到高峰。IL-17可能是参与支气管哮喘急性发作的主要细胞因子之一。

Objective To investigate the changes of the levels of IL-17 in bronchoalveolar lavage fluid of asthmatic mouse.Methods Sixty-four BALB/c mice were randomly divided into two groups,normal group and asthma group,with 32 in each group.The BALF of two groups were respectively collected after modeling on day 3,7,14,21,each time 8 rats.Lung tissues of mice at different time points of asthma group and normal group were observed by HE staining.The content of IL-17 was detected by ELISA method in BALF.Results Inflammatory responses in lung tissue of asthmatic group co mpared with normal group were obvious.IL-17 contents in BALF of asthma group were increased significantly than normal group,and the difference was statistically significant （P ＜ 0.05）.The content of IL-17 reached the peak on the 7th day after modaling.Over the same period mouse lung tissue HE staining was the most obvious inflammation.The content of IL-17 in asthmatic mice increased,and the inflammation in lung tissues of mice was the most obvious with HE staining at the same period.Conclusion The content of IL-17 in the BALF of asthma group increased,and reached the peak at the 7th day after modeling.IL-17 may be one of the main cytokines involved in acute attack of bronchial asthma.