摘要
用抗氧剂和螯合剂组合的方法 ,成功地从太白红杉的叶组织中提取和纯化了总 DNA,并对其产率、质量和纯度作了鉴定。用此方法能有效地去除酚类化合物、多糖、萜类化合物和未知化合物的干扰 ,为太白红杉的分子生物学研究提供了一套迅速、经济和可靠的技术方案。
An extraction medium with the combination of antioxidants and chelants, which can remove phenolic compound, polysaccharide and terpenoid, was successfully used to obtain total DNA from the leaf tissues of Larix chinensis beissn. The DNA yields, quality and purity were characterized. These isolated DNA could be used directly for PCR reaction, RFLP and RAPD analysis. A rapid, inexpensive and reliable procedure has been developed for further study of genetic diversity of this species.
出处
《西北大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第1期53-56,共4页
Journal of Northwest University（Natural Science Edition）
关键词
太白红杉
总DNA
抗氧剂
螯合剂
组合提取
RAPD
随机扩增多态性DNA
Larix Chinensis beissn
total DNA extraction
an extraction medium with the combination of antioxidants and chelants
RAPD(Rahdorn Amplified Polymorphism DNA)
