川牛膝多糖对口蹄疫疫苗受免小鼠树突状细胞成熟的影响

Effects of the polysaccharide from the Radix Cyathulae offcinalis Kuan(RC) on maturation of dendritic cells(DCs) from the mice immunized with FMD vaccine

用提取的川牛膝多糖(RCPS)和O型口蹄疫灭活疫苗协同免疫ICR小鼠,研究了其对免疫小鼠体内树突状细胞成熟的影响,进而探讨了RCPS增强口蹄疫疫苗免疫应答的机制。试验鼠在二免后2周处死,无菌取脾,制备脾细胞悬液,通过流式细胞术测定了共刺激信号分子(CD40,CD80,CD86)的表达水平,同时应用实时定量PCR检测了MHCⅠ、MHCⅡ类分子,CXC型趋化因子受体4(CXCR4)和CC趋化因子受体7(CCR7)mRNA表达水平。结果显示,与对照组相比在二免后14d,RCPS明显增强了共刺激信号分子CD40,CD80,CD86的表达水平,同时提高了MHCⅠ,MHCⅡ,CXCR4和CCR7mRNA表达水平。综上所述,RCPS配合FMD疫苗免疫小鼠,可显著促进树突状细胞的成熟和迁移。

This study was designed to investigate the effects of the polysaccharide from the Radix Cyathulae officinalis Kuan （RCPS） on maturation of the dendritic cells （DCs） from the mice immunized with FMDV vaccine. Spleen was collected on day 14 after boosting from sacrificed mice under aseptic conditions.The Expression of cosimulatory signaling molecules （CD40 ＋, CD80 ＋ and CD86＋） on DCs were measured by the FACSCalibur.the mRNA level of major histocompatibility complex Ⅰ （MHC Ⅰ）, major histocompatibility complex Ⅱ （MHC Ⅱ）, CXC chemokine receptor4 （CXCR4） and CC chemokine receptor7（CCR7） were detected by Real-Time PCR. In this study, Compared to mice immunized with FMDV vaccine alone, the addition of the RCPS enhanced the expression of CD40＋, CDSO＋ and CD86＋ on DCs. In addition, RCPS could up-regulated the mRNA level of MHCⅠ , MHC Ⅱ , CXCR4, CCR7. Taken together, these results demonstrate that RCPS as adjuvant could stimulate DCs maturation effectively.