猪瘟活疫苗(传代细胞源)免疫猪的攻毒保护试验

Protection of Swine against virulent Classical Swine Feuer virus using live Vaccine(Calline Propagation)

为进一步证明猪瘟活疫苗(传代细胞源)在猪场实际使用的免疫效力,本试验将中试生产的5批疫苗在广东的广州、韶关地区各选一个猪场进行临床应用试验。按照20日龄首免,60日龄二免的免疫程序,并分别于二免后第28天和第90天从5批疫苗免疫猪中各抽取5头,连同条件相同的对照猪各5头,在广东永顺生物制药股份有限公司负压动物舍进行猪瘟强毒攻击,观察发病死亡情况,评估猪瘟活疫苗(传代细胞源)在猪场实际应用的免疫保护效果。试验结果显示,5批疫苗免疫猪均能有效抵抗猪瘟强毒的攻击,既无死亡,也无猪瘟临床症状。表明在临床条件下应用猪瘟活疫苗(传代细胞源)免疫猪能产生很好的免疫保护力。

To further evaluate the efficacy of the classical swine fever live vaccine （cell line origin） in the field, this experimentwas designed to clinically test 5 batches of the vaccine in two separate pig farms located in Guangzhou and Shaoguan of the Guangdang province. Following the immunization schedule of the first one on 20-day-old and the second one on 60-day-old piglets, 5 randomly selected piglets were given each batch of the total 5 batches of vaccines, and together with those selected fromthe control group were challenged on day 28 and 90 post the second inoculation with the classical swine fever virulent strain in the animal holding facility of Guangdong Winsun Bio-pharmaceutical Co. The observation of morbidity, mortality of the testing animal and the vaccine efficacy showed that all 5 batches of the vaccine could effectively protect the piglets from the challenges of the classical swine fever virutentstrain, because there were no clinical symptoms and no death on these vaccinated animals.In summary under the clinical conditions, the classical swine fever live vaccine （cell line origin）can provide the immunized piglets with a good protection.