冬虫夏草菌丝体水提醇沉物体外抗肿瘤活性研究

Anti-tumor activity of water extracts from Cordyceps sinensis mycelia in vitro

目的研究冬虫夏草菌丝体水提醇沉物的体外抗肿瘤活性及其机制。方法采用热水浸提-醇沉法得到水提物,高效液相色谱仪测定水提醇沉物中腺苷的量;采用MTT法测定其抗肿瘤活性,利用流式细胞仪结合碘化丙锭染色法检测其对细胞周期的抑制。结果实验表明水提醇沉物能抑制人肝癌HepG2细胞株及大细胞肺癌NCI-H460细胞株的增殖,并呈浓度相关性;半数抑制浓度(IC50)值分别为(1.49±0.19)和(1.67±0.27)mg/mL。细胞周期分析表明,水提醇沉物分别阻滞HepG2及NCI-H460细胞周期于G2/M期、S期,并可诱导上述两种细胞发生凋亡。结论冬虫夏草水提醇沉物通过阻滞HepG2及NCI-H460细胞周期循环,诱导其凋亡,从而表现出良好的增殖抑制活性。为深入研究冬虫夏草菌丝体水提醇沉物抗肿瘤的机制提供了实验证据。

Objective To study the anti-tumor activity of water extract from artificially propagated Cordyceps sinensis mycelia in vitro and its mechanisms. Methods Water extract from C. sinensis mycelia was obtained using hot-water-extraction combined with alcohol precipitation method and the content of adenosine was determined using HPLC; MTT assay was performed to assess the cell growth inhibition, and the distribution of cell cycle was determined by flow cytometric analysis ofpropidium iodide staining. Results It was found that the water extract of C. sinensis mycelia could inhibit human hepatoma HepG2 cells and large cell lung cancer NCI-H460 cells growth in a concentration-dependent manner; the IC50 values were （1.49 ＋ 0.19） and （1.67 ~ 0.27） mg/mL, respectively. Cell cycle analysis showed that water extract arrested HepG2 cell cycle at GJM phase, and arrested NCI-H460 cell cycle at S phase; Furthermore, the water extract could induce the above two cell lines apoptosis. Conclusion The water extract from C. sinensis rnycelia could inhibit HepG2 and NCI-H460 cells growth through cell cycle arrest and apoptosis induction. This study provides the important experimental evidence which would contribute to the further study on the anti-tumor mechanisms of the water extract from C. sinensis mycelia.