基于成分敲除技术辨识灯心草抗焦虑的主要有效成分

Recognition of anxiolytic constituents from Juncus effusus based on constituents knock-out technique

目的明确灯心草Juncus effusus抗焦虑作用的主要有效成分。方法利用硅胶柱色谱技术,对灯心草干燥茎髓部80%乙醇提取物的醋酸乙酯超声萃取粗提物中量较高的菲类成分去氢厄弗酚、厄弗酚、去氢灯心草酚、灯心草酚进行敲除,制备相应的菲类目标成分、目标成分阴性样品,将菲类目标成分、等比例的目标成分阴性样品和全成分分别进行小鼠高架十字迷宫试验。结果菲类目标成分与全成分能明显提高小鼠进入开放臂的时间和次数,作用强度无差异,而目标成分阴性样品未表现出抗焦虑活性。结论菲类成分为灯心草抗焦虑作用的主要有效成分,灯心草中与菲类成分共存的其他成分对其抗焦虑作用基本无影响。

Objective To clarify the main anxiolytic components from Juneus effusus, Methods Silica gel column chromatography was used to extract the stem pith of J. effuses by 80% ethanol. The ethy lacetate extracts of J. effuses by ultraphonic extraction were separated to be phenanthrene constituents, such as dehydroeffusol, effusol, dehydrojuncusol, and juncusol. The phenanthrenes were knocked out and the target phenanthrene constituents and negative samples were prepared. The anxiolytic effects of the two separated fractions and the whole extract were evaluated by elevated plus-maze test in mice. Results In the elevated plus-maze test, phenanthrene constituents and the whole ethylacetate extract could significantly improve the time and number of times of mice into the open arms, without difference in the intensity of action, but no anxiolytic activity was shown in the negative sample. Conclusion Phenanthrene constituents are the main anxiolytic components in J. effusus. Other constituents in the ethylacetate extract of J, effusus do not influence the anxiolytic effect ofphenanthrene constituents.