粤蛇葡萄的化学成分及其抗血管生成活性研究

Chemical constituents from Ampelopsis cantoniensis and their anti-angiogenic activities

目的对粤蛇葡萄Ampelopsiscantoniensis地上部分的化学成分及其抗血管生成活性进行研究。方法采川多种托色谱技术进行分离纯化，通过波谱分析鉴定化合物结构，并用斑马龟模型筛选其抗血管生成活性。结果从粤蛇葡萄地上部分90％乙醇提取物的醋酸乙酯萃取部分中分离得到15个化合物，分别鉴定为粤蛇葡萄醇（1）、圆柚酮（2）、aromadendrane-4B，1013．diol（3）、脱落酸（4）、12-oxo—hardwickiicacid（5）、白桦脂酸（6）、platanicacid（7）、香草酸（8）、h黎芦醉（9）、nectandrinB（10）、nectandrinA（11）、3，5，7-三羟基色原酮（12）、5，7，3’，4‘，5’．五羟基：：氧黄酮（13）、化腆松系（14）、杨梅营（15）。结论化合物1为新的桉烷倍半萜，命名为粤蛇葡萄醇；化合物2～7、10～12为首次从蛇葡萄植r}一分离僻剑；其余化合物为首次从该植物中分离得到。化合物9、11、12表现出一定的抗斑马鱼血管生成活性。

Objective To study the chemical constituents from Ampelopsis cantoniensis and their anti-angiogenic activities. Methods The compounds were isolated and purified by various chromatographic techniques and their structures were elucidated by spectral analysis. The anti-angiogenic activities of the compounds isolated were evaluated using a zebrafish model. Results Fifteen compounds were obtained from the ethyl acetate fraction in the 90% ethanol extract of A. cantoniensis and their structures were identified as cantonienol （1）, nootkatone （2）, aromadendrane-4[3, 10[3-diol （3）, abscisic acid （4）, 12-oxo-hardwickiic acid （5）, betulinic acid （6）, platanic acid （7）, vanillic acid （8）, resveratrol （9）, nectandrin B （10）, nectandrin A （11）, 3, 5, 7-trihydroxychromone （12）, 5, 7, 3＇, 4＇, 5＇-pentahydroxyflavanone （13）, taxifolin （14）, and myricitrin （15）. Conclusion Compound 1 is a new sesquiterpene named cantonienol. Compounds 2--7 and 10--12 are isolated from the plants of Ampelopsis Michaux for the first time, and the other compounds are firstly reported in this plant. Compounds 9, ll, and 12 exhibit the weak anti-angiogenic activity when evaluated using a zebrafish model.