摘要
目的:评价结核分枝杆菌复合抗原ESAT-6/CFP-10/Rv0315在活动性肺结核诊断中的应用价值。方法:选取初治肺结核病患者70例和健康体检者34例作为结核组和健康对照组,分离PBMCs,分别用复合抗原ESAT-6/CFP-10/Rv0315、ESAT-6肽段库和CFP-10肽段库作为抗原进行ELISPOT实验,检测斑点形成细胞数。结果:复合抗原ESAT-6/CFP-10/Rv0315、ESAT-6肽段库和CFP-10肽段库检测结核分枝杆菌感染的阳性率依次为85.7%(60/70)、77.1%(54/70)、72.9%(51/70),特异性依次为82.4%(28/34)、85.3%(29/34)、88.2%(30/34)。结论:复合抗原ESAT-6/CFP-10/Rv0315作为抗原建立的ELISPOT检测方法在活动性肺结核的辅助诊断中有较高的应用价值。
Aim：To evaluate the application value of multiple antigens ESAT-6/CFP-10/Rv0315 in the detection of active pulmonary tuberculosis .Methods：A total of 70 patients with initial pulmonary tuberculosis and 34 healthy persons were enrolled as tuberculosis group and control group .PBMCs isolated from two groups were co-cultured with ESAT-6/CFP-10/Rv0315 , ESAT-6 and CFP-10 antigens .SFCs were enumerated by ELISPOT .Results： The sensitivity of ESAT-6/CFP-10/Rv0315, ESAT6 and CFP-10 antigens by ELISPOT was 85.7%（60/70）, 77.1%（54/70）, and 72.9%（51/70）, respectively.Specificity was 82.4%（28/34）, 85.3%（29/34）, and 88.2%（30/34）, respectively.Conclusion：Multiple antigens ESAT-6/CFP-10/Rv0315 showed certain value for auxiliary diagnosis of active pulmonary tuberculosis .
出处
《郑州大学学报（医学版）》
CAS
北大核心
2014年第2期211-214,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
"十二.五"国家科技重大专项2013ZX10003002-005
国家自然科学基金81271794
上海市科委科技支撑项目12441903300
河南省卫生厅科技攻关项目201203163
