饮食干预对高脂高糖妊娠大鼠肝组织中TRB3和Akt mRNA的表达及胰岛素抵抗的影响

Effects of dietary intervention on TRB3 and Akt mRNA expressions in hepatic tissue and insulin resistance of pregnant rats with high sucrose and fat diet

目的:观察饮食干预对高脂高糖妊娠大鼠肝组织中Tribbles同源蛋白3(TRB3)和Akt mRNA的表达及胰岛素抵抗的影响。方法:75只健康雌性SD大鼠随机分为高脂高糖妊娠组、高脂高糖非妊娠组、普通饮食妊娠组、普通饮食非妊娠组及高脂高糖饮食妊娠后普通饮食干预组(干预组)5组,每组15只。妊娠第20天,用全自动生化检测仪检测FBG,ELISA试剂盒测定FINS,由此计算HOMA-IR。应用RT-PCR测定大鼠肝组织中TRB3和Akt mRNA的表达水平。结果:妊娠、高脂高糖饮食均可致大鼠HOMA-IR升高(F妊娠=2 318.491,F饮食=2 888.237,F交互=993.094,P均<0.001),同时大鼠肝组织中TRB3 mRNA的表达水平升高(F妊娠=256.887,F饮食=1 749.406,F交互=2.579,P均<0.001),Akt mRNA的表达水平降低(F妊娠=221.091,F饮食=1 416.984,F交互=28.918,P均<0.001)。高脂高糖妊娠组、干预组和普通饮食妊娠组HOMA-IR、TRB3和Akt mRNA的表达水平差异均有统计学意义(F=2 772.745、445.896和390.334,P均<0.001);干预组HOMA-IR、TRB3 mRNA的表达水平较高脂高糖妊娠组降低,但仍高于普通饮食妊娠组,Akt mRNA的表达水平较高脂高糖妊娠组升高,但仍低于普通饮食妊娠组(P<0.05)。结论:TRB3和Akt可能参与了妊娠期胰岛素抵抗的发生发展,饮食干预可改善TRB3/Akt通路,减轻妊娠期胰岛素抵抗。

Aim： To study the effects of dietary intervention on the expressions of tribbles homologous protein 3 （ TRB3） and Akt mRNA in hepatic tissue and insulin resistance （ IR） of pregnant rats with high sucrose and fat diet .Meth-ods：Seventy-five healthy female SD rats were divided into five groups randomly： high sucrose and fat diet pregnant rat （HP） group,high sucrose and fat diet virgin rat（HV） group,normal diet pregnant rat（NP） group, normal diet virgin rat （NV） group and dietary intervention pregnant rat （GHP） group.Twenty days after gestation,fasting plasma glucose and fasting insulin were measured ,and IR index （ HOMA-IR） was calculated accordingly .RT-PCR was performed to detect the expressions of TRB3 and Akt mRNA in hepatic tissue .Results：Gestation and high sucrose and fat diet could cause the in-crease of HOMA-IR and TRB3 mRNA（ HOMA-IR：Fgestation =2 318.491,Fdiet =2 888.237, Finteraction =993.094,all P〈0.001;TRB3 mRNA：Fgestation =256.887,Fdiet =1 749.406, Finteraction =2.579,all P〈0.001）,and the decrease of Akt mRNA（Fgestation =221.091,Fdiet =1 416.984, Finteraction =28.918,all P〈0.001）.HOMA-IR and the levels of TRB3 mR-NA and Akt mRNA among HP group , NP group and GHP group had significant differences .HOMA-IR and the level of TRB3 mRNA in GHP group were lower than those of HP group , but still higher than those of NP group; the level of Akt mRNA was higher than that of HP group , but still lower than that of NP group （all P〈0.05）.Conclusion：TRB3 and Akt might be involved in the IR in gestation .Dietary intervention could decrease IR by improving TRB 3/Akt signal path .