卵巢上皮性癌组织中SOX2和CyclinD1的表达

Expressions of SOX2 and CyclinD1 in epithelial ovarian cancer tissue

目的:检测卵巢上皮性癌组织中SOX2和CyclinD1的表达。方法:分别采用免疫组化SP法和RT-PCR法检测20例正常卵巢上皮组织、20例卵巢良性上皮性肿瘤组织及43例卵巢上皮性癌组织中SOX2和CyclinD1蛋白及mRNA的表达。结果:3种组织间SOX2、CyclinD1蛋白及mRNA的表达差异有统计学意义(蛋白:χ2=32.998,mRNA:F=454.222、398.284,P均<0.05);卵巢上皮性癌组织中SOX2、CyclinD1蛋白及mRNA的表达高于卵巢良性上皮性肿瘤组织及正常卵巢上皮组织(P均<0.05)。SOX2、CyclinD1蛋白的表达均与卵巢上皮性癌FIGO分期、分化程度及淋巴结转移有关(P均<0.05)。卵巢上皮性癌组织中SOX2、CyclinD1蛋白的表达有关联(rp=0.422,P<0.05)。结论:SOX2和CyclinD1在卵巢上皮性癌组织中高表达,二者可能协同参与卵巢上皮性癌的发生及发展。

Aim：To explore the expressions and clinical significance of SOX 2 and CyclinD1 in epithelial ovarian canc-er tissue.Methods：Immunohistochemical SP method and RT-PCR were used to separately detect the expressions of SOX 2 and CyclinD1 in 20 cases of normal epithelial ovarian tissue , 20 cases of benign epithelial ovarian neoplasm tissue , and 43 cases of epithelial ovarian cancer tissue .Results：The expressions of SOX2 and CyclinD1 protein and mRNA in epithelial ovarian cancer tissue were significantly higher than those in benign epithelial ovarian neoplasm tissue and normal epithelial ovarian tissue（protein：χ^2 =32.998;mRNA：F=454.222,398.284, all P〈0.05）.The expressions of SOX2 and CyclinD1 protein in epithelial ovarian cancer tissue were related to FIGO stage , histological differentiation and lymph node metastasis （P〈0.05）.The expression of SOX2 protein was associated with that of CyclinD1 protein in epithelial ovarian cancer tissue （rp =0.422,P〈0.05）.Conclusion： SOX2 and CyclinD1 are both over-expressed in epithelial ovarian cancer tissue . SOX2 may be in coordinated with CyclinD 1 in carcinogenesis and progression of epithelial ovarian cancer .