摘要
采用氨基枉为固定相,乙腈/水为流动相和示差折光检测的高效液相色谱法,在乙腈/水组成比为78∶22的等组分洗脱条件下,20分钟内,可同时测定七种糖,即鼠李糖,果糖,葡萄糖,蔗糖,麦芽糖,乳糖和棉子糖;并应用这一方法,测定了猕猴桃鲜果中糖含量。分析结果表明,猕猴桃中主要糖成分是果糖、葡萄糖和蔗糖,而其总含糖量约为7～10％。
The saccharide was separated and determined by HPLC with amino bonded-column as stationary phase, acetonitrile-water as mobile phase,and detecting with refractive index detector. The seven kinds of saccharide,i.e. rhamnose, fructose, glucose, sucrose, maltose, lactose and raffinose, were determined simultaneously within 20 minutes when the ratio of the acetonitrile-water was 78:22. Through applying this method to determine the saccharide in Actinidia Chinensis Phanch fresh fruits, the determined results showed that their saccharic components are up to about 7—10％, and the main components are fructose, glucose and sucrose.
出处
《贵州科学》
1991年第2期145-148,160,共5页
Guizhou Science
关键词
HPLC法
猕猴桃
糖
食品分析
测定
saccharide
Actinidia Chinensis Phanch
determination with HPLC
