OsmiR156过表达对水稻分蘖数与生长生理相关性的影响

Effects of OsmiR156 Overexpression on the Relationship between Tiller Number and Growth Physiology of Oryza sativa L.

[目的]通过比较水稻OsmiR156组成型过表达材料D18p、OsmiR156茎秆特异性过表达材料Ubi1及其亲本日本晴分蘖期的生理生化特性,为产量性状的遗传改良提供依据。[方法]设计随机土壤盆栽试验,测定分蘖期的生物学特性。[结果]分蘖初期,3种材料的分蘖数和株高没有显著差异(P>0.05)。在分蘖中后期D18p和Ubi1的分蘖数高于日本睛,而株高低于日本睛。在分蘖后期,3种材料中Ubi1的净光合速率和吲哚乙酸氧化酶活性最高;D18p和Ubi1的叶片生长素含量和脱落酸含量都高于日本晴。[结论]OsmiR156过表达会引起植株矮化,分蘖数增多,3种材料的分蘖数与其叶片生长素含量及吲哚乙酸氧化酶活性呈线性正相关。

[Objective] Through compare biochemical characteristics of OsmiR156 constitutive overexpression rice material D18p,OsmiR156 stem specificity expression rice material Ubi1 and their parent Nipponbare in physiological at tillering stage,this will provide reference basis for genetic improvement of yield traits in rice.[Method] Random soil pot experiment was set up,and rice biological characteristics of tillering stage were determined.[Result] The tiller number and plant height of three materials are no significant difference at early tillering stage.Tiller number of D18p or Ubi1 is higher than Nipponbare,but plant height of D18p or Ubi1 is lower than Nipponbare at mid and late tillering stage.The Ubi1 leaf net light photosynthetic rate and leaf indole acetic acid oxidase activity are the highest among the three materials.The leaf auxin content and abscisic acid content in D18p and Ubi1 are higher than those in Nipponbare.[Conclusion] OsmiR156 overexpression result in plants dwarfed and tillering number increased in rice.There is positive linear relationship between the tiller number and leaf auxin content in 3 materials,and there is too positive linear relationship between tiller number and IAA oxidase activity.