转基因拟南芥中雌激素诱导的转录激活系统诱导条件的优化

Optimization of Protein Expression Condition of Estrogen-induced Transactivator( XVE) System in Transgenic Arabidopsis

[目的]探索雌激素诱导的转录激活系统(XVE)在拟南芥中高效表达外源蛋白的条件。[方法]构建XVE-AtNF-YA10:3×flag植物表达载体,转化拟南芥并获得了阳性植株;采用3种不同的诱导表达条件对外源蛋白的表达量进行检测。[结果]1/2MS培养基生长10 d后的拟南芥转基因苗外源融合蛋白表达量最高,进一步研究表明1/2MS培养基生长10 d后的拟南芥转基因苗在受诱导后8、16、24和32 h外源融合蛋白均有表达,在16 h时表达量达到最高,并持续到32 h。[结论]AtNF-YA10:3×flag融合外源基因最优表达条件为1/2MS培养基生长10 d后的拟南芥转基因苗雌激素诱导16 h。

[Objective] To optimize the protein expression using an estrogen-induced transcriptional activation system.[Method] The plasmid XVE-AtNF-YA10：3 × flag was constructed and transferred into Arabidopsis,and the positive plants were obtained.Three approaches were used to test the protcin expression level.[Results] The data showed that the protein expression level came to a head in ten-day old transgenic Arabidopsis on the 1/2 MS medium,and the protein was induced to express 8 hour later,and reach a peak from 16 hours to 32 hours.[Conclusion] The best condition for AtNF-YA10：3 × flag protein expression iu Arabidopsis was ten-day old Arabidoosis induced for 16 hours.