GC-MS法分析粗齿冷水花石油醚部位的脂溶性成分

Analysis of Liposoluble Components of Petroleum Ether Extract from Pilea sinofasciata C. J. Chen by GC-MS

[目的]研究粗齿冷水花石油醚部位中的脂溶性成分。[方法]采用硅胶柱层析分离粗齿冷水花石油醚部位脂溶性的化学成分,甲酯化后利用气相-质谱方法对其进行分析。结果:分离出25个成分,并鉴定出其中14个成分,主要成分为7,11-二甲基-3-亚甲基-(E)-1,6,10-十二碳三烯(23.750%)、1,2,3,5,6,7,8,8a-八氢-1,8a-二甲基-7-(1-甲基乙烯基)-[1S-(1α,7α.,8aα)]-萘(15.541%)、1-甲基-4-(5-甲基-1-亚甲基-4-己烯基)-(S)-环己烯(30.349%)和角鲨烯(11.485%)。[结论]这些成分均首次从粗齿冷水花中分离鉴定,为充分开发和利用该药材资源提供科学依据。

[Objective] To study the liposoluble components of petroleum ether extract from Pilea sinofasciata C.J.Chen.[Method] The liposoluble components of petroleum extract ether from Pilea sinofasciata C.J.Chen were isolated by silicagel column chromatography and analyzed by GC-MS.[Result] 25 compounds were separated and 14 of them were identified.The main ingredients were 7,11-dimethyl-3-methylene-（E）-1,6,10-dodecatriene （23.75％）,1,2,3,5,6,7,8,8a-octahydro-1,8a-dimethyl-7-（1-methylethenyl）-[1S-（1α,7α,8aα）]-naphthalene（15.541％）,1-methyl-4-（5-methyl-1-methylene-4-hexenyl）-（S）-cyclohexene （30.349％）,squalene （11.489％）.[Conclusion] All the compounds were isolated from the plant for the first time.The results provide profitable supplements and references for the further utilization and study of Pilea sinofasciata C.J.Chen.