双链蛋白聚糖对胃癌细胞生长作用研究

Effect of biglycan on growth of gastric cancer cells

目的:研究双链蛋白聚糖(biglycan,BGN)对人胃癌细胞系SGC-7901的细胞增殖、周期及凋亡等生长作用影响。方法:构建重组质粒pIRES2-EGFP/BGN,转染胃癌细胞株SGC-7901,获得胃癌稳转细胞株SGC-7901/BGN。通过细胞增殖实验(CCK-8法)、平板克隆实验、流式细胞技术,分别检察BGN过表达对SGC-7901细胞增殖、克隆形成、周期及凋亡的影响。结果:SGC-7901/BGN组胃癌细胞较SGC-7901/空载组生长明显抑制(P<0.01),细胞培养板形成的克隆数明显减少[(209.7±12.6)比(326.0±15.1)];同时过表达BGN可促进胃癌细胞的凋亡[(10.7±1.5)%比(5.3±1.1)%],且将细胞周期阻止在G1期[(55.7±2.1)%比(37.6±1.8)%]。结论:胃癌细胞过表达BGN可抑制胃癌细胞的增殖、克隆形成。BGN可能通过将细胞周期阻滞在G1期、诱导细胞凋亡,从而发挥其生长抑制的生物学效应。

Objective To investigate the effect of biglycan （BGN） on the proliferation, cycle and apoptosis in gastric cancer cell lines SGC-7901. Methods The recombinant plasmid pIRES2-EGFP/BGN was created and transfected into gas- tric cancer cell lines SGC-7901 to generate the cell lines SGC-7901/BGN overexpressing BGN stably. The effect of BGN on cell proliferation, colony formation, cycle and apoptosis was investigated by CCK-8 assay, colony formation assay and flow cytometry. Results The proliferation of SGC-7901 cells transfected with BGN was inhibited more than that transfected with vector （P〈0.01）. The number of clones formed in the cell culture plate is also significantly reduced [（209.7±12.6） vs （326.0±15.1）. The colony formation of SGC-7901/BGN cells in culture medium decreased with that of SGC-7901/vector. BGN induced gastric cancer cells apoptosis [（10.7±1.5）% vs （5.3±1.1）%] and arrest cell cycle in G1 phase [（55.7±2.1）% vs （37.6±1.8）%]. Conclusions Overexpression of BGN in gastric cancer cells can inhibit cell proliferation and colony forma- tion, arrest the cell cycle in G1 phase and induces apoptosis. Key words： Gastric cancer; Biglycan; Growth