老年黄斑变性患者与对照人群膳食、血清叶黄素/玉米黄素以及血脂水平的比较

Comparison of daily intake of lutein + zeaxanthin,serum concentration of lutein /zeaxanthin and lipids profile between age-related macular degeneration patients and controls

目的:比较老年黄斑变性(age-related macular degeneration,AMD)患者与对照人群膳食和血清中叶黄素、玉米黄素及血脂水平。方法:根据眼底形态学及年龄相关性眼病研究(age-related eye disease study,AREDS)的标准对AMD进行诊断与分级,募集早期AMD患者、中期AMD患者及非AMD对照各51人。采用食物频率调查法,分析膳食叶黄素+玉米黄素的每日摄入量;采用高效液相色谱法测定血清叶黄素及玉米黄素水平;采用罗氏全自动生化分析仪测定血脂(总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白)水平。结果:中期AMD组膳食叶黄素+玉米黄素摄入量、血清叶黄素及玉米黄素水平分别为7 870.458μg/d、0.180μmol/L、0.029μmol/L,均低于对照组(11 297.959μg/d、0.285μmol/L、0.044μmol/L,P<0.05);早、中期AMD组血清高密度脂蛋白水平分别为(1.29±0.27)mmol/L、(1.16±0.30)mmol/L,均低于对照组[(1.45±0.35)mmol/L,P<0.001]。结论:膳食叶黄素+玉米黄素的摄入量和血清叶黄素、玉米黄素水平可能影响AMD的发展进程,血清高密度脂蛋白水平可能与AMD的发生相关。

Objective：To compare the daily intake of lutein ± zeaxanthin, serum concentrations of lutein, zeaxanthin and serum lipids between age-related macular degeneration （AMD） patients and controls. Methods： AMD was diagnosed and graded according to the fundus morphology and the standard of agerelated eye disease study （AREDS）. In the study, 51 subjects with early AMD, 51 with medium AMD and 51 without AMD as controls were recruited. Food frequency questionnaires were used to calculate the daily intake of lutein and zeaxanthin. The concentrations of serum lutein and zeaxanthin were measured by HPLC and the concentrations of serum lipids including serum total cholesterol （TC）, triglyceride （TG）, high density lipoprotein （I-IDL） and low density lipoprotein （LDL） were measured by Roche full- automatic biochemical analyzer. Results： The daily intake of lutein ± zeaxanthin, the concentrations of serum lutein and serum zeaxanthin of the subjects with medium AMD were 7 870. 458 μg/d, 0. 180 p.mol/L, 0. 029 μmol/L respectively, which were all significantly lower than those of the controls （ 11 297. 959 μg/d, 0. 285 μmol/L, 0. 044 μmol/L, P 〈 0.05 ） ; The concentrations of serum HDL of the subjects with early and medium AMD were （ 1.29 ± 0.27 ） mmol/L and （ 1.16 ± 0.30） mmol/L respectively, both of which were significantly lower than that of the controls [ （ 1.45 ± 0.35 ） mmol/L, P 〈 0. 001 ]. Conclusion： The development of AMD might be affected by the daily intake of lutein ＋ zeaxanthin and the serum concentrations of lutein and zeaxanthin. The concentration of serum HDL might be related to the occurrence of AMD.