人牙周膜间充质干细胞的分离培养及超微结构观察

Isolation,culture and ultrastructure analysis of mesenchymal stem cells from human periodontal ligament

目的:分离培养人牙周膜间充质干细胞并观察其超微结构。方法:组织块法原代培养人牙周膜细胞,克隆培养观察细胞克隆形成情况,采用STRO-1为标记物以免疫磁珠法分离筛选人牙周膜间充质干细胞,瑞氏-姬姆萨染色观察细胞形态,透射电镜观察分离筛选细胞的超微结构。结果:人牙周膜干细胞具有克隆形成能力,胞浆内核糖体丰富,可见粗面内质网和线粒体,而其他胞浆细胞器较少,具有处于低分化细胞超微结构特点。结论:牙周膜干细胞具有克隆形成能力和一般干细胞的超微结构特点。

Objective： To isolate and culture human periodontal ligament stem cells （PDLSCs） and observe its uhrastructure. Methods： The proliferation and growth characteristics of human periodontal ligament cells were observed in primary culture and colony culture. PDLSCs were isolated by magnetic activated cell sorting （MACS） and ultrastructural characterization was observed by electron microscopy. Results： When the cells were cultured at low density, PDLSCs grew in a colony-like manner. With the exception of a small amount of rough endoplasmic reticulum, ribosomes, and mitochondria, relatively few organelles were found in the cytoplasm, suggesting that they had remained undifferentiated. Conclusion： PDLSCs showed colony-like growth capacity and had ultrastructural characterization with stem cells. This indicated that PDLSCs could act as the appropriate seed cells for cell-based periodontal tissue regeneration.