双须骨舌鱼性腺发育的组织学观察

Histological study on the gonadal development of Osteoglossum bicirrhosum

通过组织学研究方法，分别对双须骨舌鱼Ⅱ～V期的卵巢及Ⅱ～Ⅵ期精巢的形态结构、特征及生殖细胞变化进行描述。结果显示，Ⅱ时相卵母细胞的细胞核较大，约为细胞体积的一半；IU时相卵母细胞出现卵黄斑及卵黄颗粒；IV时相卵母细胞中卵黄迅速积累且细胞膜出现褶皱；V时相卵母细胞膜表面的褶皱消失。雄性双须骨舌鱼精巢发育的Ⅱ期，只含有初级精母细胞。Ⅲ期精巢分布有初级和次级精母细胞，精小管形成且小管中充满精细胞；Ⅳ期精巢主要含有初级和次级精母细胞以及精原细胞，精小管中出现空腔；V期精巢中包含次级精母细胞、精原细胞和精子细胞，精小管中的空腔增大；VI期精巢则显示出高度血管化和结缔组织增多等特点。本研究将为双须骨舌鱼人工繁殖提供参考。

The histology of gonadal development of Osteoglossum bicirrhosum which is a popular ornamental fish was studied by paraffin section technique and H. E staining method. The morphological characteristics and structures of ovary at stage 1I - V and testis at stage II - VI were described. Moreover, the development of oocyte and spermatid were also described. The results showed that four months old ovaries were in stage I1 , while the oocytes were in phase ]I , and the nucleus occupied the half part of the cell. Eight months to two years old ovaries were in stage 1I ,most of the oocytes were in phase II1 and egg macular and yolk granules could be observed;two to three years old ovaries were in stage IV, with the oocytes in phase IV which had yolk substance accumulated quickly and oolemma drape appeared. The ovary in stage V was three years or more than three years old, and the oocytes in phase V were full of yolk granules with the drape of oolemma disappeared. The primary spermatocyte in testis could be seen at the stage I1. Eight months to two years old testes were in stage 11I, while the secondary spermatocyte appeared and the seminiferous tubules took shapes, and spermatids are full of the seminiferous tubules, primary spermatocyte, secondary spermatocyte and spermatids were seen at the stage IV, while the seminiferous tubules of void appeared. The testis in stage V was three years old and more than three years old, the spermatozoa appeared and the void of seminiferous tubules became large. The testis highly vascularized and with connective tissue increased was data for the artificial reproduction of also observed which was in stage VI. This study will provide the basic O. bicirrhosum.